Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis

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<center><h1>Protocol Overview</h1></center>
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<center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/>
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<b>To check PCR results use High melt gel</b><br/><br/>
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What you need:<br/>
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- High-melt agarose<br/>
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- TAE buffer 1X<br/><br/>
 +
 
 +
Procedure:<br/>
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1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/>
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2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
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3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/>
 +
 
 +
<b>To do a gel purification use Low melt gel</b><br/><br/>
 +
What you need:<br/>
 +
- Low-melt agarose<br/>
 +
- TAE buffer 1X<br/><br/>
 +
Procedure:<br/>
 +
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/>
 +
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/>
 +
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/>
 +
 
 +
 
 +
 
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Revision as of 19:36, 1 June 2012

Header

Protocols

Select Protocol

Making Gel Electrophoresis gel solutions



To check PCR results use High melt gel

What you need:
- High-melt agarose
- TAE buffer 1X

Procedure:
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box)
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved 3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

To do a gel purification use Low melt gel

What you need:
- Low-melt agarose
- TAE buffer 1X

Procedure:
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED

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