From 2012.igem.org
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| - | <h1>Weeks</h1>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w2">2</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w3">3</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w4">4</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w5">5</a></td>
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| - | <td>6</td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w7">7</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w8">8</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w9">9</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w10">10</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w11">11</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w12">12</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w13">13</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w14">14</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w15">15</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w16">16</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w17">17</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w18">18</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w19">19</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w20">20</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w21">21</a></td>
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| - | <td><a href="https://2012.igem.org/Team:Evry/Notebook/w22">22</a></td>
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| - | </tr>
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| | <h1>Week 6: 16th July - 22nd July</h1> | | <h1>Week 6: 16th July - 22nd July</h1> |
Latest revision as of 09:59, 3 August 2012
Weeks:
| June |
July |
August |
September |
October |
November |
Week 6: 16th July - 22nd July
Monday, 16th July
Bacterial Transformation:
Transformation of AID + TIR1 containing plasmid into TOP10 bacterias.
Thuesday, 17th July
Reception and preparation of pCS2+ primers.
Friday, 20th July
PCR Colony:
PCR colony of T10 pSC2+ with RFP
Primers used : SP6 and T3
Site Mutagenesis directed:
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
Primer used : SdMTIR1-Fw and SdMTIR1-Rv
"
