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Team:Evry/Notebook/w6
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Revision as of 21:01, 2 August 2012
Weeks
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 |
Week 6: 16th July - 22nd July
Monday, 16th July
Bacterial Transformation:
Transformation of AID + TIR1 containing plasmid into TOP10 bacterias.Thuesday, 17th July
Reception and preparation of pCS2+ primers.Friday, 20th July
PCR Colony:
PCR colony of T10 pSC2+ with RFPPrimers used : SP6 and T3
Site Mutagenesis directed:
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions SitePrimer used : SdMTIR1-Fw and SdMTIR1-Rv
