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Template:Team:Edinburgh/Notebook/Week06
From 2012.igem.org
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Half cell voltage increased 10-fold to 50 mV<br/><br/> | Half cell voltage increased 10-fold to 50 mV<br/><br/> | ||
<b> PCR for Shewanella </b><br/> | <b> PCR for Shewanella </b><br/> | ||
| - | Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB. <br/> | + | Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB. <br/> |
| + | <img style="width:350px;" src="https://www.evernote.com/shard/s109/res/1d418ac4-cfba-4a11-91d5-e437016fd7c3/SkitchImage0.png" /><img style="width:350px;" src="https://www.evernote.com/shard/s109/res/6c924f34-ee1b-4d1e-95f1-bb53471e7559/SkitchImage0.png" /> | ||
</p> | </p> | ||
Revision as of 11:01, 1 August 2012
Half-cell results
Half cell voltage increased 10-fold to 50 mV
PCR for Shewanella
Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB.
Kod PCR for Shewanella
Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day.
Half-cell experiment
2 new half-cells were assembled and set for autoclaving
PCR for Shewanella
Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA sample from previous raction was purified.
