Template:Team:Edinburgh/Notebook/Week06

From 2012.igem.org

Week 6, 30.07.2012, Monday

Half-cell results
Half cell voltage increased 10-fold to 50 mV

PCR for Shewanella
Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB.

Week 6, 31.07.2012, Tuesday

Kod PCR for Shewanella
Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day.

Half-cell experiment
2 new half-cells were assembled and set for autoclaving

Week 6, 01.08.2012, Wednesday

PCR for Shewanella
Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA, mtrA, mtrCAB samples were purified.


Week 6, 02.08.2012, Thursday

Week 6, 03.08.2012, Friday