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Team:NTNU Trondheim/Stocks
From 2012.igem.org
(Difference between revisions)
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(→Isolated DNA) |
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|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||pSB1C3 | |VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||pSB1C3 | ||
|- | |- | ||
| - | |RFP +Term||11.07|| || | + | |RFP +Term||11.07||<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> ||pSB2K3 |
|- | |- | ||
| - | |Constitutive promoter||22.06|| ||27,7|| | + | |Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||pSB1A2 |
|- | |- | ||
| - | |DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8|| | + | |DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8||BBa_J63010 |
|- | |- | ||
| - | |K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8|| | + | |K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8||pSB1A2 |
|- | |- | ||
| - | |LuxR+HSL||24.06|| || || | + | |LuxR+HSL||24.06||<partinfo>BBa_R0062</partinfo> || ||pSB1A2 |
|- | |- | ||
| - | |pBAD+lysis||9.7|| ||23,6|| | + | |pBAD+lysis||9.7|| ||23,6||pSB1A3 |
|- | |- | ||
|DNA lysis||24.06|| || || | |DNA lysis||24.06|| || || | ||
Revision as of 18:59, 28 July 2012
This page lists stored cultures and other samples:
See also Constructs page.
Resuspended DNA
(Dried DNA from distribution kit resuspended in dH2O)
| Name/abbreviation | Date stored | Description | Source | Backbone |
|---|---|---|---|---|
| LR | ? | <partinfo>BBa_C0062</partinfo> | (RS) | pSB1A2 |
| Lysis | 22.06 | BBa_K112808 | (RS) | pSB1A2 |
| C78 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | |
| C79 | ? | <partinfo>BBa_C0079</partinfo> | (RS) | |
| RBS | 24.06 | <partinfo>BBa_B0034</partinfo> | (RS) | pSB1A2 |
| LuxI | ? | <partinfo>BBa_C0061</partinfo> | (RS) | |
| K+RBS | ? | <partinfo>BBa_K081005</partinfo> | (RS) | pSB1A2 |
Isolated DNA
(Miniprep, gel extraction and PCR products)
| Name/abbreviation | Date stored | Description | Concentration | Backbone |
|---|---|---|---|---|
| LuxR+Term | 16.7 | BBa_C0062 + BBa_B0015 | 37,0 | pSB1A2 |
| LuxI+Term | 16.7 | BBa_C0061 + BBa_B0015 | 37,6 | pSB1A2 |
| C79 | 27.06 | <partinfo>BBa_C0079</partinfo> | 70,8 | pSB2K3 |
| C78 | 27.06 | <partinfo>BBa_C0078</partinfo> | 63,4 | pSB2K3 |
| RBS | 27.06 | <partinfo>BBa_B0034</partinfo> | 30,2 | pSB1A2 |
| GFP+Term | 11.07 | BBa_K082003 + BBa_B0015 | pSB1AK3 | |
| CFP+Term | 11.07 | BBa_E0020 + BBa_B0015 | pSB1A2 | |
| LI (BBa_K092400) | ? | <partinfo>BBa_K092400</partinfo> | pSB1A2 | |
| YFP+Term | 11.07 | YFP+term (religated) | ? | |
| DLD + RBS | 9.07 | BBa_K284003 + BBa_B0034 | 24,1 | |
| VHB + RBS | 9.07 | BBa_K258005 + BBa_B0034 | 48,3 | |
| Jen1 + RBS | 9.07 | BBa_K284002 + BBa_B0034 | 35,3 | |
| VGB + RBS | 9.07 | BBa_K561001 + BBa_B0034 | 40,0 | |
| LR | ? | <partinfo>BBa_B0062</partinfo> | 20,3 | |
| VGB promoter | 27.06 | <partinfo>BBa_K561001</partinfo> | 22,9 | pSB1C3 |
| VGB DNA | 25.06 | <partinfo>BBa_K561001</partinfo> | pSB1C3 | |
| RFP +Term | 11.07 | <partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | pSB2K3 | |
| Constitutive promoter | 22.06 | <partinfo>BBa_J23119</partinfo> | 27,7 | pSB1A2 |
| DLD | 4.07 | <partinfo>BBa_K284003</partinfo> | 63,8 | BBa_J63010 |
| K+RBS | 13.07 | <partinfo>BBa_K081005</partinfo> | 19,8 | pSB1A2 |
| LuxR+HSL | 24.06 | <partinfo>BBa_R0062</partinfo> | pSB1A2 | |
| pBAD+lysis | 9.7 | 23,6 | pSB1A3 | |
| DNA lysis | 24.06 | |||
| D2 | 22.6 | BBa_K145150 | 23,1 | pSB1A2 |
| RBS | 6.07 | <partinfo>BBa_B0034</partinfo> | 44,5 | pSB1A2 |
| LuxI | 6.07 | 38,6 | ||
| VHB | 4.07 | <partinfo>BBa_K258005</partinfo> | 44,9 | pSB1A2 |
| Jen | 4.07 | <partinfo>BBa_K284002</partinfo> | 102,1 | |
| luxR+HSL | 25.06 | 13,3 | ||
| Lysis1 DNA | 24.06 | |||
| GFP+DTT | 13.07 | BBa_K082003+BBa_B0015 | 41,1 | pSB1AK3 |
| YFP+DTT | 13.07 | BBa_E0030+BBa_B0015 | 55,5 | pSB1AK3 |
| RFP+DTT | 13.07 | BBa_E1010+BBa_B0015 | 40,9 | pSB2K3 |
| CFP+DTT | 13.07 | BBa_E0020+BBa_B0015 | 47,7 | pSB1A2 |
| pBAD B1 | 1.07 | <partinfo>Bba_K206000</partinfo> | 24,0 | pSB1A3 |
| D1 | 22.06 | <partinfo>BBa_K091146</partinfo> | 19,8 | pSB1A2 |
| pBAD B2 | 1.07 | <partinfo>Bba_K206000</partinfo> | 19 | pSB1A3 |
| Colicin | 4.07 | <partinfo>BBa_K150009</partinfo> | 58,6 | |
| VGB+RBS+YFP+DTT | 26.07 | pSB1A2 | ||
| VHB+RBS+YFP+DTT | 26.07 | pSB1A2 | ||
| EYFP+RBS+YFP+DTT | 26.07 | |||
| pTet GFP | 26.07 | <partinfo>BBa_I13522</partinfo> | pSB1A2 | |
| RBS | 26.06 | <partinfo>BBa_B0034</partinfo> | 16,8 | pSB1A2 |
| DTT Amp | 26.06 | <partinfo>BBa_B0015</partinfo> | 35,8 | pSB1AK3 |
| DTT Kan | 26.06 | <partinfo>BBa_B0015</partinfo> | 26,5 | pSB1AK3 |
| RFP K | 22.06 | BBa_E1010 | pSB2K3 | |
| GFP AK | 22.06 | BBa_K082003 | pSB1AK3 | |
| CFP A | 22.06 | BBa_E0020 | pSB1A2 | |
| YFP AK | 22.06 | BBa_E0030 | pSB1AK3 |
Glycerol stocks
(Cell cultures kept at -80 C)
| Name/abbreviation | Date stored | Strain | Plasmid | Position |
|---|---|---|---|---|
| pTet mRFP (BBa_I13521) | 27.07 | DH5a | pSB1A2 | |
| pTet GFP (BBa_I13522) | 27.07 | DH5a | pSB1A2 | |
| EYFP + LVA (BBa_E0430) | 27.07 | DH5a | pSB1A2 | |
| RFP (<partinfo>BBa_E1010</partinfo>) | 18.07 | DH5a | pSB2K3 | |
| VGB (<partinfo>BBa_K561001</partinfo>) | 18.07 | DH5a | pSB1C3 | |
| LuxR+HSL (<partinfo>BBa_R0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| Lysis (<partinfo>BBa_K112808</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| pBAD (<partinfo>Bba_K206000</partinfo>) | 16.07 | DH5a | pSB1A3 | |
| LuxR (<partinfo>BBa_C0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| GFP (<partinfo>BBa_K082003</partinfo>) | 16.07 | DH5a | pSB1AK3 | |
| pBAD+Lysis | 16.07 | DH5a | pSB1A3 | |
| DTT (<partinfo>BBa_B0015</partinfo> | 17.07 | DH5a | pSB1AK3 | |
| D2 (<partinfo>BBa_K145150</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| CFP (<partinfo>BBa_E0020</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| YFP (<partinfo>BBa_E0030</partinfo>) | 17.07 | DH5a | pSB1AK3 |
