Team:NTNU Trondheim/Stocks

From 2012.igem.org

(Difference between revisions)
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See also [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs page].
See also [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs page].
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Resuspended DNA (dried DNA from distribution kit resuspended in dH2O)
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===Resuspended DNA===
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(Dried DNA from distribution kit resuspended in dH2O)
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{| class="wikitable"
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Isolated DNA (Miniprep, gel extraction and PCR products):
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! Name/abbreviation ||  Date stored ||  Description  || Source|| Backbone
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|-
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|LR||? ||<partinfo>BBa_C0062</partinfo>||(RS)||pSB1A2
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|-
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|Lysis||22.06 ||BBa_K112808||(RS)||pSB1A2
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|-
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|C78||?||<partinfo>BBa_C0078</partinfo>||(RS)||
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|-
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|C79||?||<partinfo>BBa_C0078</partinfo>||(RS)||
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|RBS||24.06||<partinfo>BBa_B0034</partinfo>||(RS)||pSB1A2
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|LuxI||?||<partinfo>BBa_C0061</partinfo>||(RS)||
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|-
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|K+RBS||?||<partinfo>BBa_K081005</partinfo>||(RS)||pSB1A2
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|}
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===Isolated DNA===
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(Miniprep, gel extraction and PCR products)
{| class="wikitable"
{| class="wikitable"
! Name/abbreviation ||  Date stored ||  Description  || Concentration|| Backbone
! Name/abbreviation ||  Date stored ||  Description  || Concentration|| Backbone
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|-
 
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|Lysis||22.06 ||BBa_K112808||(RS)||pSB1A2
 
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|LuxR+Term||16.7|| || ||
|LuxR+Term||16.7|| || ||
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|CFP+Term||11.07|| || ||
|CFP+Term||11.07|| || ||
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|LR||? ||<partinfo>BBa_C0062</partinfo>||(RS)||pSB1A2
 
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|LI||?||<partinfo>BBa_K092400</partinfo>||
|LI||?||<partinfo>BBa_K092400</partinfo>||
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|C78||?||<partinfo>BBa_C0078</partinfo>||(RS)||
 
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|-
 
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|C79||?||<partinfo>BBa_C0078</partinfo>||(RS)||
 
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|RBS||24.06||<partinfo>BBa_B0034</partinfo>||(RS)||pSB1A2
 
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|YFP+Term||11.07||?|YFP+term (religated)||?||
|YFP+Term||11.07||?|YFP+term (religated)||?||
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|CFP+DTT||13.07|| ||47,7||
|CFP+DTT||13.07|| ||47,7||
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|LuxI||?||<partinfo>BBa_C0061</partinfo>||(RS)||
 
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|pBAD B1||1.07||<partinfo>Bba_K206000</partinfo>||24,0||
|pBAD B1||1.07||<partinfo>Bba_K206000</partinfo>||24,0||
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|D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||pSB1A2
|D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||pSB1A2
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|K+RBS||?||<partinfo>BBa_K081005</partinfo>||(RS)||pSB1A2
 
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|pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||pSB1A3
|pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||pSB1A3
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|}
|}
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===Glycerol stocks===
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(Cell cultures kept at -80 C)
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Glycerol stocks:
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{| class="wikitable"
{| class="wikitable"

Revision as of 15:17, 28 July 2012

This page lists stored cultures and other samples:

See also Constructs page.

Resuspended DNA

(Dried DNA from distribution kit resuspended in dH2O)

Name/abbreviation Date stored Description Source Backbone
LR? <partinfo>BBa_C0062</partinfo>(RS)pSB1A2
Lysis22.06 BBa_K112808(RS)pSB1A2
C78?<partinfo>BBa_C0078</partinfo>(RS)
C79?<partinfo>BBa_C0078</partinfo>(RS)
RBS24.06<partinfo>BBa_B0034</partinfo>(RS)pSB1A2
LuxI?<partinfo>BBa_C0061</partinfo>(RS)
K+RBS?<partinfo>BBa_K081005</partinfo>(RS)pSB1A2

Isolated DNA

(Miniprep, gel extraction and PCR products)

Name/abbreviation Date stored Description Concentration Backbone
LuxR+Term16.7
LuxI+Term16.7
C7927.06<partinfo>BBa_C0079</partinfo>70,8pSB2K3
C7827.06<partinfo>BBa_C0078</partinfo>63,4pSB2K3
RBS27.06<partinfo>BBa_B0034</partinfo>30,2pSB1A2
GFP+Term11.07
CFP+Term11.07
LI?<partinfo>BBa_K092400</partinfo>
YFP+Term11.07YFP+term (religated)?
DLD + RBS9.07 24,1
VHB + RBS9.07 48,3
Jen1 + RBS9.07 35,3
VGB + RBS9.07 40,0
LR?<partinfo>BBa_B0062</partinfo> 20,3
VGB promoter27.06<partinfo>BBa_K561001</partinfo>22,9pSB1C3
VGB DNA25.06<partinfo>BBa_K561001</partinfo> pSB1C3
RFP +Term11.07
Constitutive promoter22.06 27,7
DLD4.07<partinfo>BBa_K284003</partinfo>63,8
K+RBS13.07<partinfo>BBa_K081005</partinfo>19,8
LuxR+HSL24.06
pBAD+lysis9.7 23,6
DNA lysis24.06
D222.6BBa_K14515023,1pSB1A2
RBS6.07<partinfo>BBa_B0034</partinfo>44,5pSB1A2
LuxI6.07 38,6
VHB4.07<partinfo>BBa_K258005</partinfo>44,9pSB1A2
Jen4.07<partinfo>BBa_K284002</partinfo>102,1
luxR+HSL25.06 13,3
Lysis1 DNA24.06
GFP+DTT13.07 41,1
YFP+DTT13.07 55,5
RFP+DTT13.07 40,9
CFP+DTT13.07 47,7
pBAD B11.07<partinfo>Bba_K206000</partinfo>24,0
D122.06<partinfo>BBa_K091146</partinfo>19,8pSB1A2
pBAD B21.07<partinfo>Bba_K206000</partinfo>19pSB1A3
Colicin4.07<partinfo>BBa_K150009</partinfo>58,6
VGB+RBS+YFP+DTT26.07
VHB+RBS+YFP+DTT26.07
EYFP+RBS+YFP+DTT26.07
pTet GFP26.07<partinfo>BBa_I13522</partinfo> pSB1A2
RBS26.06<partinfo>BBa_B0034</partinfo>16,8pSB1A2
DTT Amp26.06<partinfo>BBa_B0015</partinfo>35,8pSB1AK3
DTT Kan26.06<partinfo>BBa_B0015</partinfo>26,5pSB1AK3
RFP K22.06
GFP AK22.06
CFP A22.06
YFP AK22.06

Glycerol stocks

(Cell cultures kept at -80 C)

Name/abbreviation Date stored Strain Plasmid Position
pTet mRFP (BBa_I13521)27.07DH5apSB1A2
pTet GFP (BBa_I13522)27.07DH5apSB1A2
EYFP + LVA (BBa_E0430)27.07DH5apSB1A2
RFP (<partinfo>BBa_E1010</partinfo>) 18.07 DH5a pSB2K3
VGB (<partinfo>BBa_K561001</partinfo>)18.07DH5apSB1C3
LuxR+HSL (<partinfo>BBa_R0062</partinfo>)16.07 DH5apSB1A2
Lysis (<partinfo>BBa_K112808</partinfo>)16.07 DH5apSB1A2
pBAD (<partinfo>Bba_K206000</partinfo>)16.07 DH5apSB1A3
LuxR (<partinfo>BBa_C0062</partinfo>)16.07 DH5apSB1A2
GFP (<partinfo>BBa_K082003</partinfo>)16.07 DH5apSB1AK3
pBAD+Lysis16.07 DH5apSB1A3
DTT (<partinfo>BBa_B0015</partinfo>17.07 DH5apSB1AK3
D2 (<partinfo>BBa_K145150</partinfo>)17.07 DH5apSB1A2
CFP (<partinfo>BBa_E0020</partinfo>)17.07 DH5apSB1A2
YFP (<partinfo>BBa_E0030</partinfo>)17.07 DH5apSB1AK3
Retrieved from "http://2012.igem.org/Team:NTNU_Trondheim/Stocks"