Team:Evry/Notebook/July/23

From 2012.igem.org

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4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.<br/>
4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.<br/>
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----
 +
PCR of pCS2+:
 +
 +
<table borderColor="#4B5670" cellSpacing="0" cellPadding="8" width="800" border="1">
 +
      <tr>
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        <td>'''Réactifs'''</td>
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        <td>'''Volumes (µl)'''</td>
 +
      </tr>
 +
      <tr>
 +
        <td>GC Buffer</td>
 +
        <td>10</td>
 +
      </tr> 
 +
      <tr>
 +
        <td>dNTPs</td>
 +
        <td>1</td>
 +
      </tr> 
 +
      <tr>
 +
        <td>DNA</td>
 +
        <td>1</td>
 +
      </tr> 
 +
      <tr>
 +
        <td>H2O</td>
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        <td>32,5</td>
 +
      </tr> 
 +
      <tr>
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        <td>Primers (FW and RV)</td>
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        <td>2,5 each</td>
 +
      </tr> 
 +
      <tr>
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        <td>Phusion polymerase</td>
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        <td>0,5</td>
 +
      </tr> 
<html>
<html>

Latest revision as of 14:59, 25 July 2012

Promoters & Reporters workgroup

4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.

PCR of pCS2+:

Réactifs Volumes (µl)
GC Buffer 10
dNTPs 1
DNA 1
H2O 32,5
Primers (FW and RV) 2,5 each
Phusion polymerase 0,5
Retrieved from "http://2012.igem.org/Team:Evry/Notebook/July/23"