Team:Evry/Notebook/July/20

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<FONT SIZE=3>'''PCR Colony'''</FONT> <br/>
<FONT SIZE=3>'''PCR Colony'''</FONT> <br/>
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PCR colony of T10 pSC2+ with RFP <br/> ''Primers used'' : SP6 and T3 </br>
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PCR colony of T10 pSC2+ with RFP <br/> Primers used : SP6 and T3 </br>
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<br/>
<br/>
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<FONT SIZE=3>'''Site Mutagenesis directed'''</FONT> <br/>
<FONT SIZE=3>'''Site Mutagenesis directed'''</FONT> <br/>
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site <br>
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site <br>
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''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv
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Primer used : SdMTIR1-Fw and SdMTIR1-Rv
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<a href="https://2012.igem.org/Team:Evry/Notebook/July/19"><img  src="https://static.igem.org/mediawiki/2012/e/ed/Previous_day_arrow.png"></a>
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Latest revision as of 17:33, 20 July 2012

Reporter + TIR1 groups

'''PCR Colony'''

PCR colony of T10 pSC2+ with RFP
Primers used : SP6 and T3


'''Site Mutagenesis directed'''
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
Primer used : SdMTIR1-Fw and SdMTIR1-Rv

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