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Team:Evry/Notebook/July/20
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<FONT SIZE=3>'''PCR Colony'''</FONT> <br/> | <FONT SIZE=3>'''PCR Colony'''</FONT> <br/> | ||
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| - | PCR colony of T10 pSC2+ with RFP <br/> | + | PCR colony of T10 pSC2+ with RFP <br/> ''Primers used'' : SP6 and T3 </br> |
<br/> | <br/> | ||
<br/> | <br/> | ||
<FONT SIZE=3>'''Site Mutagenesis directed'''</FONT> <br/> | <FONT SIZE=3>'''Site Mutagenesis directed'''</FONT> <br/> | ||
| - | PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site | + | PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site </br> |
| + | ''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv | ||
<center> | <center> | ||
<a href="https://2012.igem.org/Team:Evry/Notebook/July/19"><img src="https://static.igem.org/mediawiki/2012/e/ed/Previous_day_arrow.png"></a> | <a href="https://2012.igem.org/Team:Evry/Notebook/July/19"><img src="https://static.igem.org/mediawiki/2012/e/ed/Previous_day_arrow.png"></a> | ||
Revision as of 17:31, 20 July 2012
Reporter + TIR1 groups
PCR colony of T10 pSC2+ with RFP
''Primers used'' : SP6 and T3
'''Site Mutagenesis directed'''
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site ''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv
