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Team:Columbia-Cooper-NYC/Making Liquid Media
From 2012.igem.org
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<LI>1.0 g of magnesium sulfate MgSO4 | <LI>1.0 g of magnesium sulfate MgSO4 | ||
<LI>0.4 g of dipotassium phosphate K2HPO4 | <LI>0.4 g of dipotassium phosphate K2HPO4 | ||
| - | <LI>5.0 mL | + | <LI>5.0 mL of MD-TMS |
</UL> | </UL> | ||
| - | <LI>Using sulfuric acid ( | + | <LI>Using sulfuric acid (H2SO4), adjust pH of solution to 2.1. (This should take about 0.5 mL of H2SO4.) |
| - | <LI>Add 20 g of FeSO4 | + | <LI>Add 20 g of FeSO4*7H20 |
<LI>Using sulfuric acid, adjust pH of solution to 1.8-1.85 | <LI>Using sulfuric acid, adjust pH of solution to 1.8-1.85 | ||
<LI>Once solution appears homogeneous, filter sterilize | <LI>Once solution appears homogeneous, filter sterilize | ||
Latest revision as of 07:47, 7 January 2013
Liquid Media Protocol
For 1 liter of liquid media:
- Combine in a large beaker, while stirring with a magnetic bar:
- 1 L of distilled water
- 0.8 g of ammonium sulfate (NH4)2SO4
- 1.0 g of magnesium sulfate MgSO4
- 0.4 g of dipotassium phosphate K2HPO4
- 5.0 mL of MD-TMS
- Using sulfuric acid (H2SO4), adjust pH of solution to 2.1. (This should take about 0.5 mL of H2SO4.)
- Add 20 g of FeSO4*7H20
- Using sulfuric acid, adjust pH of solution to 1.8-1.85
- Once solution appears homogeneous, filter sterilize
Credit for protocol goes to Jason Candreva, Research Associate, Columbia University
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