Team:KAIST Korea/Notebook Labnote/2012 10 temp
From 2012.igem.org
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<section id="10"> | <section id="10"> | ||
<div class="date">October 10<sup>th</sup> 2012</div></br> | <div class="date">October 10<sup>th</sup> 2012</div></br> | ||
- | <div id="content_note" > | + | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> |
- | < | + | |
+ | <div class="note-title">LuxI and Xis OE template preparation</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1010Fig1" src="https://static.igem.org/mediawiki/2012/3/39/KAIST_LABNOTE101012_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
</br> | </br> | ||
+ | </div> | ||
+ | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
+ | |||
+ | <div class="note-title">pAutoIntegrase cloning check and Xis template vector prep</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1010Fig2" src="https://static.igem.org/mediawiki/2012/4/4f/KAIST_LABNOTE101012_Fig2.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little">pAutoIntegrase 1-10 – HindIII cut</br></span> | ||
+ | <span id="little">pGEM-Xis 1-2 – EcoRI cut</br></span> | ||
+ | |||
+ | |||
+ | </br> | ||
</div> | </div> | ||
+ | |||
+ | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
+ | |||
+ | <div class="note-title">insert DNA preparation with OE PCR</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1010Fig3" src="https://static.igem.org/mediawiki/2012/d/df/KAIST_LABNOTE101012_Fig3.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little">┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)</span></br> | ||
+ | |||
+ | </br> | ||
+ | <div align="center"><img id="figure" alt="2_1010Fig4" src="https://static.igem.org/mediawiki/2012/7/7d/KAIST_LABNOTE101012_Fig4.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little">┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)</span> | ||
+ | |||
+ | </br> | ||
+ | </div> | ||
+ | |||
+ | |||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="11"> | <section id="11"> | ||
<div class="date">October 11<sup>th</sup> 2012</div></br> | <div class="date">October 11<sup>th</sup> 2012</div></br> | ||
- | <div id=" | + | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> |
- | + | ||
- | + | ||
+ | <div class="note-title">Additional templates</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1011Fig1" src="https://static.igem.org/mediawiki/2012/2/2d/KAIST_LABNOTE101112_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align='center'> | ||
+ | <span id="little">┗ Integrase and Excisionase template</span></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
</div> | </div> | ||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="14"> | <section id="14"> | ||
<div class="date">October 14<sup>th</sup> 2012</div></br> | <div class="date">October 14<sup>th</sup> 2012</div></br> | ||
- | <div id=" | + | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> |
- | + | ||
- | + | ||
+ | <div class="note-title">pAutoIntegrase Cloning</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <span id="little">Insert DNA – OE PCR</span> | ||
+ | </br></br> | ||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1014Fig1" src="https://static.igem.org/mediawiki/2012/1/12/KAIST_LABNOTE101412_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align='center'> | ||
+ | <span id="little">Cloning into pSB4A5 is performed with gel extracted DNA fragment.</span></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
</div> | </div> | ||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="16"> | <section id="16"> | ||
<div class="date">October 16<sup>th</sup> 2012</div></br> | <div class="date">October 16<sup>th</sup> 2012</div></br> | ||
+ | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
+ | |||
+ | <div class="note-title">pAutoSimple inserts nested PCR and aiiA template</div> | ||
+ | |||
<div id="content_note" > | <div id="content_note" > | ||
- | |||
- | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1016Fig1" src="https://static.igem.org/mediawiki/2012/c/cb/KAIST_LABNOTE101612_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
</div> | </div> | ||
+ | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
+ | |||
+ | <div class="note-title">pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1016Fig2" src="https://static.igem.org/mediawiki/2012/c/c4/KAIST_LABNOTE101612_Fig2.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little">single digestion with HindIII.</span> | ||
+ | |||
+ | </br> | ||
+ | </div> | ||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="17"> | <section id="17"> | ||
<div class="date">October 17<sup>th</sup> 2012</div></br> | <div class="date">October 17<sup>th</sup> 2012</div></br> | ||
+ | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
+ | |||
+ | <div class="note-title">pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation</div> | ||
<div id="content_note" > | <div id="content_note" > | ||
- | < | + | |
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1017Fig1" src="https://static.igem.org/mediawiki/2012/9/9e/KAIST_LABNOTE101712_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
</br> | </br> | ||
+ | |||
+ | <span id="little">sizes ok</span> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
+ | |||
+ | <div class="note-title">pAuto insert DNA preparation</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1017Fig2" src="https://static.igem.org/mediawiki/2012/5/56/KAIST_LABNOTE101712_Fig2.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align='center'> | ||
+ | <span id="little">intermediate fragment nested PCR</span></div> | ||
+ | |||
+ | </br> | ||
+ | <div align="center"><img id="figure" alt="2_1017Fig3" src="https://static.igem.org/mediawiki/2012/7/79/KAIST_LABNOTE101712_Fig3.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align='center'> | ||
+ | <span id="little">Full pAuto insert DNA construct OE PCR</span></div> | ||
+ | |||
+ | </br> | ||
+ | |||
</div> | </div> | ||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="18"> | <section id="18"> | ||
<div class="date">October 18<sup>th</sup> 2012</div></br> | <div class="date">October 18<sup>th</sup> 2012</div></br> | ||
- | + | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | |
- | + | ||
- | + | ||
+ | <div class="note-title">pAutoIntegrase enz cut check with NotI</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1018Fig1" src="https://static.igem.org/mediawiki/2012/6/66/KAIST_LABNOTE101812_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little">Lane 3, 6, 8 seem to be cloned.</span> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
</div> | </div> | ||
+ | |||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="19"> | <section id="19"> | ||
<div class="date">October 19<sup>th</sup> 2012</div></br> | <div class="date">October 19<sup>th</sup> 2012</div></br> | ||
+ | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
+ | |||
+ | <div class="note-title">pAutoIntegrase Alternative sequencing with fragments amplification</div> | ||
+ | |||
<div id="content_note" > | <div id="content_note" > | ||
- | |||
- | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1019Fig1" src="https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE101912_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
</div> | </div> | ||
- | + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | |
- | + | ||
- | + | ||
</section> | </section> | ||
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<section id="24"> | <section id="24"> | ||
<div class="date">October 24<sup>th</sup> 2012</div></br> | <div class="date">October 24<sup>th</sup> 2012</div></br> | ||
- | <div id=" | + | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> |
- | + | ||
- | + | ||
+ | <div class="note-title">OE template preparation</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1024Fig1" src="https://static.igem.org/mediawiki/2012/9/96/KAIST_LABNOTE102412_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align = 'center'> | ||
+ | <span id="little">gp47, aiiA, LuxI, bFMO for simple and pAuto</span></div> | ||
+ | |||
+ | </br> | ||
+ | <div align="center"><img id="figure" alt="2_1024Fig1" src="https://static.igem.org/mediawiki/2012/5/52/KAIST_LABNOTE102412_Fig2.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align = 'center'> | ||
+ | <span id="little">promoter and Inverted one</span></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
</div> | </div> | ||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
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<section id="25"> | <section id="25"> | ||
<div class="date">October 25<sup>th</sup> 2012</div></br> | <div class="date">October 25<sup>th</sup> 2012</div></br> | ||
- | <div id="content_note" > | + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> |
- | < | + | <div class="note-title">pAuto intermediate fragment OE PCR</div> |
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="1025Fig1" src="https://static.igem.org/mediawiki/2012/f/f1/KAIST_LABNOTE102512_Fig1.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
+ | |||
+ | <div class="note-title">OE PCR with new primer</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | |||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_1025Fig2" src="https://static.igem.org/mediawiki/2012/a/a9/KAIST_LABNOTE102512_Fig2.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
</br> | </br> | ||
+ | <div align='center'> | ||
+ | <span id="little">pAutoSimple/inverted</span></div> | ||
+ | </br> | ||
+ | <div align="center"><img id="figure" alt="2_1025Fig2" src="https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE102512_Fig3.png"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | <div align='center'> | ||
+ | <span id="little">pAuto/inverted</span></div> | ||
+ | |||
+ | </br> | ||
</div> | </div> | ||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
+ | |||
</section> | </section> | ||
<section id="26"> | <section id="26"> | ||
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</html> | </html> | ||
{{:Team:KAIST_Korea/footer}} | {{:Team:KAIST_Korea/footer}} | ||
+ | <!-- sample | ||
+ | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
+ | |||
+ | <div class="note-title">TITLE</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <span id="little">CONTENT</span> | ||
+ | </br></br> | ||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_08##Fig#" src="#"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little"></span> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
+ | |||
+ | <div class="note-title">TITLE2</div> | ||
+ | |||
+ | <div id="content_note" > | ||
+ | |||
+ | <span id="little">CONTENT2</span> | ||
+ | </br></br> | ||
+ | <b>Results</b></br></br> | ||
+ | |||
+ | <div align="center"><img id="figure" alt="2_08##Fig#" src="#"></img></div> | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | <span id="little"></span> | ||
+ | |||
+ | </br> | ||
+ | </div> | ||
+ | --> |
Revision as of 01:55, 27 October 2012
![](https://static.igem.org/mediawiki/2012/5/59/Logopart_Kaist.png)
![](https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png)
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Notebook : Labnote-October
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
![](https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png)
Labnote
OctoberOctober 1st 2012
No Special Event!
Back to the CalendarOctober 2nd 2012
No Special Event!
Back to the CalendarOctober 3rd 2012
No Special Event!
Back to the CalendarOctober 4th 2012
No Special Event!
Back to the CalendarOctober 5th 2012
No Special Event!
Back to the CalendarOctober 6th 2012
No Special Event!
Back to the CalendarOctober 7th 2012
No Special Event!
Back to the CalendarOctober 8th 2012
No Special Event!
Back to the CalendarOctober 9th 2012
No Special Event!
Back to the CalendarOctober 10th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
LuxI and Xis OE template preparation
Results
![2_1010Fig1](https://static.igem.org/mediawiki/2012/3/39/KAIST_LABNOTE101012_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
pAutoIntegrase cloning check and Xis template vector prep
Results
pAutoIntegrase 1-10 – HindIII cut
pGEM-Xis 1-2 – EcoRI cut
![2_1010Fig2](https://static.igem.org/mediawiki/2012/4/4f/KAIST_LABNOTE101012_Fig2.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
insert DNA preparation with OE PCR
Results
┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)
┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)
Back to the Calendar![2_1010Fig3](https://static.igem.org/mediawiki/2012/d/df/KAIST_LABNOTE101012_Fig3.png)
![2_1010Fig4](https://static.igem.org/mediawiki/2012/7/7d/KAIST_LABNOTE101012_Fig4.png)
October 11th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
Additional templates
Results
Back to the Calendar![2_1011Fig1](https://static.igem.org/mediawiki/2012/2/2d/KAIST_LABNOTE101112_Fig1.png)
┗ Integrase and Excisionase template
October 12th 2012
No Special Event!
Back to the CalendarOctober 13th 2012
No Special Event!
Back to the CalendarOctober 14th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoIntegrase Cloning
Insert DNA – OE PCR
Results
Back to the Calendar![2_1014Fig1](https://static.igem.org/mediawiki/2012/1/12/KAIST_LABNOTE101412_Fig1.png)
Cloning into pSB4A5 is performed with gel extracted DNA fragment.
October 15th 2012
No Special Event!
Back to the CalendarOctober 16th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoSimple inserts nested PCR and aiiA template
Results
![2_1016Fig1](https://static.igem.org/mediawiki/2012/c/cb/KAIST_LABNOTE101612_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut
Results
single digestion with HindIII.
Back to the Calendar![2_1016Fig2](https://static.igem.org/mediawiki/2012/c/c4/KAIST_LABNOTE101612_Fig2.png)
October 17th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation
Results
sizes ok
![2_1017Fig1](https://static.igem.org/mediawiki/2012/9/9e/KAIST_LABNOTE101712_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
pAuto insert DNA preparation
Results
Back to the Calendar![2_1017Fig2](https://static.igem.org/mediawiki/2012/5/56/KAIST_LABNOTE101712_Fig2.png)
intermediate fragment nested PCR
![2_1017Fig3](https://static.igem.org/mediawiki/2012/7/79/KAIST_LABNOTE101712_Fig3.png)
Full pAuto insert DNA construct OE PCR
October 18th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoIntegrase enz cut check with NotI
Results
Lane 3, 6, 8 seem to be cloned.
Back to the Calendar![2_1018Fig1](https://static.igem.org/mediawiki/2012/6/66/KAIST_LABNOTE101812_Fig1.png)
October 19th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoIntegrase Alternative sequencing with fragments amplification
Results
Back to the Calendar![2_1019Fig1](https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE101912_Fig1.png)
October 20th 2012
No Special Event!
Back to the CalendarOctober 21st 2012
No Special Event!
Back to the CalendarOctober 22nd 2012
No Special Event!
Back to the CalendarOctober 23rd 2012
No Special Event!
Back to the CalendarOctober 24th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
OE template preparation
Results
Back to the Calendar![2_1024Fig1](https://static.igem.org/mediawiki/2012/9/96/KAIST_LABNOTE102412_Fig1.png)
gp47, aiiA, LuxI, bFMO for simple and pAuto
![2_1024Fig1](https://static.igem.org/mediawiki/2012/5/52/KAIST_LABNOTE102412_Fig2.png)
promoter and Inverted one
October 25th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAuto intermediate fragment OE PCR
Results
![1025Fig1](https://static.igem.org/mediawiki/2012/f/f1/KAIST_LABNOTE102512_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
OE PCR with new primer
Results
Back to the Calendar![2_1025Fig2](https://static.igem.org/mediawiki/2012/a/a9/KAIST_LABNOTE102512_Fig2.png)
pAutoSimple/inverted
![2_1025Fig2](https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE102512_Fig3.png)
pAuto/inverted
October 26th 2012
No Special Event!
Back to the CalendarOctober 27th 2012
No Special Event!
Back to the CalendarOctober 28th 2012
No Special Event!
Back to the CalendarOctober 29th 2012
No Special Event!
Back to the CalendarOctober 30th 2012
No Special Event!
Back to the CalendarOctober 31st 2012
No Special Event!
Back to the Calendar