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Team:KAIST Korea/Notebook Labnote/2012 10
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Revision as of 21:46, 26 October 2012
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Notebook : Labnote-October
Labnote
OctoberOctober 1st 2012
No Special Event!
Back to the Calendar
Back to the CalendarOctober 2nd 2012
No Special Event!
Back to the Calendar
October 3rd 2012
Flip FlopBxb1 double transformants vector miniprep & PCR check
Results
Discussions
Seemed to every colonies have appropriate vectors.
Back to the Calendar
October 4th 2012
No Special Event!
Back to the Calendar
October 5th 2012
No Special Event!
Back to the Calendar
October 6th 2012
No Special Event!
Back to the Calendar
October 7th 2012
No Special Event!
Back to the Calendar
October 8th 2012
No Special Event!
Back to the Calendar
October 9th 2012
No Special Event!
Back to the Calendar
October 10th 2012
Flip FlopBxb1_GTG_pBAD single cut check with different enzyme
Single cut with SnaBI
Results
No desired size(5.6kb) vector appeared.
Back to the Calendar
Flip Flop
Flip Flop
Flip Flop
Flip Flop
October 11th 2012
No Special Event!
Back to the Calendar
October 12th 2012
Flip FlopBxb1_GTG_pBAD colony #4~7 inoculation, miniprep and single cut check
Single cut with SnaBI
Single cut with SnaBI
Results
Cloning completed
Back to the Calendar
October 13th 2012
No Special Event!
Back to the Calendar
October 14th 2012
No Special Event!
Back to the Calendar
October 15th 2012
Flip FlopBBa_C0060, BBa_C0061, BBa_C0062 transformation into TOP10
Templates for Auto-regulated FlipFlop project.
Back to the Calendar
October 16th 2012
Flip FlopBBa_C0060, BBa_C0061, BBa_C0062, bFMO inoculation and enzyme cut check
BBa_C0060, BBa_C0061, BBa_C0062: from colony, EcoRI single cut
Back to the Calendar
- BBa_C0060: 2859bp
- BBa_C0061: 2688bp
- BBa_C0062: 2826bp
- bFMO: from cell stock, EcoRI single cut
October 17th 2012
Flip FlopTemplate preparation for OE PCR
Promoter construct: 210bp
Results
Promoter PCR failed
Back to the Calendar
Flip Flop
Flip Flop
Flip Flop
Flip Flop
Flip Flop
October 18th 2012
Flip FlopTemplate preparation for OE PCR
Results
Terminator PCR failed
Back to the Calendar
October 19th 2012
Flip FlopTemplate preparation for OE PCR
Terminator: 179bp
Results
→ Gel extracted
Back to the Calendar
October 20th 2012
Flip FlopTemplate preparation for OE PCR
Bxb1 Xis: 777bp
Results
bFMO PCR w/ various condtions
Bxb1_Xis-pGEM_B1 transfomation
Synthesized gene – Bxb1_Xis arrived and transformed into TOP10
Back to the Calendar
October 21st 2012
No Special Event!
Back to the Calendar
October 22nd 2012
Flip FlopOverlapping extension PCR
Results
→ Gel extracted
Back to the Calendar
October 23rd 2012
Flip FlopOverlapping extension PCR-Nested PCR
Results
Discussions
There was too little full construct nested. We think this is due to primer interfering by its LVA homology.
Overlapping extension PCR 2
Results
Discussions
Bands were more intense than those of first trial.
Back to the Calendar
October 24th 2012
No Special Event!
Back to the Calendar
October 25th 2012
No Special Event!
Back to the Calendar
October 26th 2012
No Special Event!
Back to the Calendar
October 27th 2012
No Special Event!
Back to the Calendar
October 28th 2012
No Special Event!
Back to the Calendar
October 29th 2012
No Special Event!
Back to the Calendar
October 30th 2012
No Special Event!
Back to the Calendar
October 31st 2012
No Special Event!
Back to the Calendar
