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Team:Freiburg/Project/Coop
From 2012.igem.org
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<b>Collaboration with Team Potsdam Bioware:</b> | <b>Collaboration with Team Potsdam Bioware:</b> | ||
| - | Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston. | + | Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid, was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston. |
Revision as of 21:32, 26 October 2012
Collaborations
Collaboration with Team Potsdam Bioware: Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid, was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston.
Collaboration with Team Fatih Medical:
We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project.
Collaboration with Team Evry:
On the regional Jamboree in Amsterdam, we were fascinated by the work of the Evry iGEM Team. Using Xenopus as a model organism for synthetic biology is clearly an interesting and challenging endeavor. We got approached by Nicolas Pollet from Team Evry, who wants to apply our GATE kit in Xenopus. That is why we currently prepare to send our kit to Team Evry.
