"
Team:UC Chile/Cyano/Labook/october
From 2012.igem.org
(Difference between revisions)
| Line 40: | Line 40: | ||
| - | Synechocystis PCC 6803 cells were transformed with BBa_K743018 and BBa_K73010. Positive colonies appeared in both plates. | + | Synechocystis PCC 6803 cells were transformed with BBa_K743018 and BBa_K73010. Positive colonies appeared in both plates.<br> |
| - | A Gibson assembly and a transformation were done to modify construct BBa_K743015. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion. | + | A Gibson assembly and a transformation were done to modify construct BBa_K743015. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.<br> |
| - | A Gibson assembly and a transformation were done to modify construct BBa_K743018. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion. | + | A Gibson assembly and a transformation were done to modify construct BBa_K743018. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.<br> |
Bioluminiscence of Synechocystis cells transformed with BBa_K743014 and BBa_K743015 was measured in a High-sensitive camera. | Bioluminiscence of Synechocystis cells transformed with BBa_K743014 and BBa_K743015 was measured in a High-sensitive camera. | ||
Latest revision as of 19:13, 26 October 2012
October
Synechocystis PCC 6803 cells were transformed with BBa_K743018 and BBa_K73010. Positive colonies appeared in both plates.
A Gibson assembly and a transformation were done to modify construct BBa_K743015. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.
A Gibson assembly and a transformation were done to modify construct BBa_K743018. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.
Bioluminiscence of Synechocystis cells transformed with BBa_K743014 and BBa_K743015 was measured in a High-sensitive camera.
