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Team:Columbia-Cooper-NYC/OD Measurement
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Latest revision as of 03:38, 4 October 2012
Optical Density Measurement Protocol
- Preset temperature and wavelength for measuring the optical density
- Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
- Place 10µl cells into 1mL solution
- Read the absorbance
Make note that when reading the optical density, must be referenced by a reference solution
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