Team:UIUC-Illinois/Results/Scaffold
From 2012.igem.org
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<img src="https://static.igem.org/mediawiki/2012/d/d4/Tether1.jpg" height=50% width=50%> | <img src="https://static.igem.org/mediawiki/2012/d/d4/Tether1.jpg" height=50% width=50%> | ||
- | <br/><b>Fig 1.</b> | + | <br/><b>Fig 1.</b> 50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 68.7oC 30 sec. annealing time, 35 cycles for amplification and 10 cycles for tethering. PUF and cCFP (lanes 2 & 6) were first amplified and then used in the tethering reaction in lane 3. |
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<img src="https://static.igem.org/mediawiki/2012/b/b1/Tether2.jpg" height=50% width=50%> | <img src="https://static.igem.org/mediawiki/2012/b/b1/Tether2.jpg" height=50% width=50%> | ||
<br/><b>Fig 2.</b> | <br/><b>Fig 2.</b> | ||
+ | 50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in HF Buffer, 68.7oC 30 sec. annealing time, 10 cycles of PCR. | ||
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<img src="https://static.igem.org/mediawiki/2012/c/cf/Tether3.jpg" height=50% width=50%> | <img src="https://static.igem.org/mediawiki/2012/c/cf/Tether3.jpg" height=50% width=50%> | ||
<br/><b>Fig 3.</b> | <br/><b>Fig 3.</b> | ||
+ | 50mL 10 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 1 min. annealing time, 10 cycles of PCR, half the volume of primers used (0.5 μL instead of 1 μL). | ||
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<img src="https://static.igem.org/mediawiki/2012/b/b6/Tether4.jpg" height=50% width=50%> | <img src="https://static.igem.org/mediawiki/2012/b/b6/Tether4.jpg" height=50% width=50%> | ||
<br/><b>Fig 4.</b> | <br/><b>Fig 4.</b> | ||
+ | |||
+ | 50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 68.7oC 30 sec. annealing time, 25 cycles of PCR. | ||
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<img src="https://static.igem.org/mediawiki/2012/f/f5/Tether5.jpg" height=50% width=50%> | <img src="https://static.igem.org/mediawiki/2012/f/f5/Tether5.jpg" height=50% width=50%> |
Revision as of 22:07, 3 October 2012