"

From 2012.igem.org

Revision as of 00:01, 1 October 2012 (view source) Bchen26 (Talk | contribs) ← Older edit		Revision as of 22:04, 3 October 2012 (view source) Bchen26 (Talk | contribs) Newer edit →
Line 31:		Line 31:
	<div id="scaffoldSelection">		<div id="scaffoldSelection">
	<center><h2>RNA Scaffold</h2></center>		<center><h2>RNA Scaffold</h2></center>
-	<li><a name="scaffold0" >RNA Scaffold Data Overview</a></li>	+	<li><a name="scaffold0" >RNA Scaffold Results Overview</a></li>
-	<li><a name="scaffold1" >Scaffold Data</a></li>	+	<li><a name="scaffold1" >RNA Scaffold Data</a></li>
	<li><a name="scaffold2" >PUF Tethering Data</a></li>		<li><a name="scaffold2" >PUF Tethering Data</a></li>
	<li><a name="scaffold3" >Conclusion</a></li>		<li><a name="scaffold3" >Conclusion</a></li>
Line 67:		Line 67:
	<br/><br/>		<br/><br/>
-	<center><img src="https://static.igem.org/mediawiki/2012/f/ff/IVT_gel.jpg" height=100% width=100%><br/>	+	<center><img src="https://static.igem.org/mediawiki/2012/a/a4/IV-T3.png" height=100% width=100%><br/>
	</center>		</center>
	<br/>		<br/>

---

Revision as of 22:04, 3 October 2012

RNA Scaffold Overview

The results covered in this section are of the experiments overviewed in the RNA Scaffold Design section.

RNA Scaffold Data

Fig. 1. 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight for WT PUF-PIN and 6-2/7-2 PUF-PIN. 2 mM IPTG induction for 2 hours. 200 mL cultures incubated at 37oC till 1 nm optical density after inoculation with 2 mL of overnight for WT PUF-αGFP cultures. 2 mM IPTG induction for various hours.


Fig. 2. 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight. 2 mM IPTG induction for 2 hours. His-Tag Ni-NTA purification, centrifuged with Millipore 30kDa cutoff ultracentrifuge tubes.


Fig. 3. 1 L culture incubated at 37oC till 0.5 nm optical density after inoculation with 5 mL of overnight. 2 mM IPTG induction for 2 hours. His-Tag Ni-NTA purification, centrifuged with Millipore 30kDa cutoff ultracentrifuge tubes.



Fig. 4. In-Vitro Transcription with MEGAscript® T7 Kit (Invitrogen)

PUF Tethering Data

Fig 1.


Fig 2.


Fig 3.


Fig 4.


Fig 5.

Conclusion