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Team:Arizona State/Notebook/hyder
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(Created page with "{{:Team:Arizona_State/Template:Header}} Hyder's lab notes 6/22/12 miniprepped gfp1 and rbs1 and rbs2 liquid cultures picked 1 colony from double terminator (dt1) plate pick...")
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Revision as of 02:30, 1 October 2012
6/22/12
miniprepped gfp1 and rbs1 and rbs2 liquid cultures
picked 1 colony from double terminator (dt1) plate
picked 1 colony from t7 polymerase (pol1) plate
picked 1 colony from puc19 plate (positive control)
picked 1 colony from dh5a plate (negative control)
started liquid cultures of each colony (5 mL LB amp each)
8/3/12
Resuspended GFPT1 and GFPT2 oligos with molecular grade (nuclease-free) H2O.
Final Concentration 100uM
(gfpt1 top1, gfpt2 top1, gfpt1 top2, gftp2 top2, gfpt1 bot1, gfpt2 bot1, gfpt1 bot2, gfpt2 bot2)
(3uL of each oligo + 2uL 10x annealing buffer, 6uL molecular grade H2O. 20uL Reactions)
Heated for 5 minutes at 100C. Let cool to room temperature on the heating block, stored at -20C.
8/3/12
