Team:Arizona State/Notebook

From 2012.igem.org

(Difference between revisions)

Revision as of 01:15, 1 October 2012

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Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.

June 07

Transformation (LSE)
- Transformed DNA:
  - lacZ (well 4:12G, I732019)
  - p + lacO (well 1:6G, R0011)
- Cells: neb10beta (donated)
- Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
- Controls: puc19, no DNA (8 plates)

June 08

Transformation results
- puc19: growth
- negative control: no growth
- lacZ, lacO: possible small colonies
liquid culture in amp media (100 ug / ml):
- no growth of lacZ, lacO
- growth of puc19

@@ Line 20: / Line 20: @@
 ==June 08==
+* Transformation results
+** puc19: growth
+** negative control: no growth
+** lacZ, lacO: possible small colonies
+* liquid culture in amp media (100 ug / ml):
+** no growth of lacZ, lacO
+** growth of puc19