Team:UIUC-Illinois

From 2012.igem.org

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<center><h2><a href="https://2012.igem.org/Team:UIUC-Illinois/Project/Future/AssemblyLine>Enzymatic Assembly Line</a></h2><br/><center>
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<p>In utilizing the versatility of the PUF based toolkit we are developing, our current research involves an engineered metabolic pathway. The characterization and standardization of these genes in biobrick format is underway. Future goals involving the PUF toolkit will focus on tethering enzymes of productive artificial or natural pathways for kinetic favorability.</p>
<p>In utilizing the versatility of the PUF based toolkit we are developing, our current research involves an engineered metabolic pathway. The characterization and standardization of these genes in biobrick format is underway. Future goals involving the PUF toolkit will focus on tethering enzymes of productive artificial or natural pathways for kinetic favorability.</p>

Revision as of 19:09, 30 September 2012

Header

Main

Mission Statement

The ultimate goal of the University of Illinois 2012 iGEM team is to construct an RNA based PUF protein toolkit which may be utilized for the manipulation of gene expression. Customizable manipulation will involve gene silencing by specific RNA scission. Also, the PUF protein's properties also allow for its use in optimizing multi-step enzymatic pathways. In this way, we aim to improve the versatility and cost efficiency of biological production systems by use of our toolkit. In developing a protein based RNA binding toolkit, researchers will be able to transmute the biobricked PUF to do what is necesary for their scientific endeavors. We seek to standardize and characterize the nature of PUF.

Click here to read our project abstract.


Our Research Outside of PUF


Aside from our main project researching the RNA specific affinity of PUF and its respective endonuclease fusion protein variants, we have also devoted time and resources into exploring and testing other potential applications for PUF. Mainly, our ancillary projects directly related to PUF include research involving an RNA scaffold and an enzymatic assembly line based on such a scaffold. Additionally, we have characterized the 2011 University of Washington iGEM team's Petrobrick (BBa_K590025).

To read more about these projects and the data we have collected, please visit their respective pages. You can reach them either through hovering over the "Ancillary" tab or by clicking the hyperlinks in this section.

    • Click above on a part of our project that you want to read about.

    Sponsors


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