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Team:UIUC-Illinois/Project/Design
From 2012.igem.org
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<center><h2>Experimental Design</h2></center> | <center><h2>Experimental Design</h2></center> | ||
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| - | <p>In designing our project we based our quantitative tests on fluorescence measured by a plate fluorescence reader. Our constructs were created in ways that best suit providing evidence for our hypothesis of the PUF-PIN fusion protein showing endonuclease activity. Generally, our results were collected from quantifying two main PUF-PIN fusion protein types, a wild type and a mutant type, in different conditions. These two fusion proteins had recognition sites that differed by one base pair. <br/><br/>Click on the list to the left to read about each of our constructs and why we decided to do them.</p | + | <p>In designing our project we based our quantitative tests on fluorescence measured by a plate fluorescence reader. Our constructs were created in ways that best suit providing evidence for our hypothesis of the PUF-PIN fusion protein showing endonuclease activity. Generally, our results were collected from quantifying two main PUF-PIN fusion protein types, a wild type and a mutant type, in different conditions. These two fusion proteins had recognition sites that differed by one base pair. <br/><br/>Click on the list to the left to read about each of our constructs and why we decided to do them.</p> |
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Revision as of 03:12, 29 September 2012
