"
Team:UANL Mty-Mexico/Notebook
From 2012.igem.org
(Difference between revisions)
JuanQuijano (Talk | contribs) |
JuanQuijano (Talk | contribs) |
||
| Line 23: | Line 23: | ||
<tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr> | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr> | ||
</table> | </table> | ||
| + | |||
| + | <p><br></p> | ||
For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab. | For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab. | ||
Revision as of 03:49, 27 September 2012
Cloning Strategy
Fusion proteins
We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.
 |
 |
