Team:UANL Mty-Mexico/Notebook/wetlab

Figure 3. Ready for the minipreps! Here are some transformed bacteria cultures, in the right we can see the clone 1 of the 1-3A+L2 genetic construction.

Figure 4. Here's a partial digest of the construction 1-3A+L2 cutted with XhoI.

Figure 5. Positive restriction digest of 1-3A+L2 and 1-3A (pSB1C3) as control vector.

Figure 6. Maps and simulation of restriction digest of p1C3-L2 and pSB1C3, simulation with XhoI.

Figure 7. 600th, not just a regular gel, 10 tracks of Cph8 (photoreceptor), 2 tracks of 1-23L (terminator), 10 tracks of S1TT (genetic contruction of a the first part Biphasic switch and terminator) and a last track of S4-GFP (second part of the Biphasic swith) all of these used for HuBac project.

Figure 8. Synthetic genes.

Figure 9. Cloning the synthetics, minipreps of he synthetics genes.

Figure 10. Subcloning the synthetic constitutive promoter into 1-3A.

Figure 11. Maps and digestions simulations of 1-3A+p21 (constitutive promoter) and 1-3A both cutted with XhoI.

Figure 12. Electrophoresis gels of rhMT clones, rhMT+L2 and 1-3A.

Figure 13. .

Figure 19. GlpF transportator cloned in pSB3T5 bacteria (white colonies)

Figure 20. GlpF transportator cloned in pSB3T5 digestion

Figure 21. High-speed double digestion in a microwave at different seconds (tracks 2-4).

Figure 22. Map of pSB1C3-RFP, and a simulation of a digestion with EcoRI and PstI.

Figure 23. A little fragment in an electrophoresis gel, aaaand it's gone.

Figure 24. As you can see, this is what we mean when we say "work optimization".