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Team:UANL Mty-Mexico/Notebook
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Revision as of 03:46, 27 September 2012
Cloning Strategy
Fusion proteins
We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.
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