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Team:UANL Mty-Mexico/Notebook
From 2012.igem.org
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| - | <p><br><h3> | + | <p><br><h3>Cloning Strategy</h3><br></p> |
<p><b>Fusion proteins</b></p> | <p><b>Fusion proteins</b></p> | ||
| - | <p>We used a cloning strategy similar to the Standard Assembly 21 based on the compatible restriction sites BglII and BamHI. | + | <p>We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.</p> |
<p><br></p> | <p><br></p> | ||
Revision as of 03:44, 27 September 2012
Cloning Strategy
Fusion proteins
We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.
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