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Team:UANL Mty-Mexico/Notebook
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<p>We used a cloning strategy similar to the Standard Assembly 21 based on the compatible restriction sites BglII and BamHI. A scar is obtained which translated result in the benefical aminoacids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allow us to use the Standard Assembly 10 backbones.</p> | <p>We used a cloning strategy similar to the Standard Assembly 21 based on the compatible restriction sites BglII and BamHI. A scar is obtained which translated result in the benefical aminoacids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allow us to use the Standard Assembly 10 backbones.</p> | ||
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<table class="image" align="center" > | <table class="image" align="center" > | ||
<caption align="bottom"><b>Figure 1.</b> Cloning strategy for contructions of fusion proteins.</caption> | <caption align="bottom"><b>Figure 1.</b> Cloning strategy for contructions of fusion proteins.</caption> | ||
| - | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/ | + | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr> |
</table> | </table> | ||
Revision as of 03:41, 27 September 2012
Notebook
Fusion proteins
We used a cloning strategy similar to the Standard Assembly 21 based on the compatible restriction sites BglII and BamHI. A scar is obtained which translated result in the benefical aminoacids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allow us to use the Standard Assembly 10 backbones.
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