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Team:TU Darmstadt/Protocols
From 2012.igem.org
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR] | ||
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA Purification of DNA] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA Purification of DNA] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest] | ||
Revision as of 21:31, 26 September 2012
Protocols
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Glycerine Stocks
- Heat Shock Transformation
- Miniprep / Fermentas kit
- Miniprep / Promega kit
- Miniprep / Qiagen kit
- Protein Expression Est13
- Protein Expression FsC
- Western blot
Comamonas testosteroni
- [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Ammonium acetate precipitation
- Cloning
- Dephosphorylation
- DNA Ligation
- DNA Ligation à la group transport
- DNA Quantification / NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Resuspension of primers
- Salting Out
- Sequencing DNA
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
In silico
Data analysis
Databases
- [http://www.rcsb.org/pdb/home/home.do PDB]
- [http://www.cas.org/ CAS]
Modelling
Sequence analysis
Structure analysis
Material Science
- Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine
- Synthesis of polyethylene terephthalate in presence of sulfuric acid
- Surface analysis of polyethylene terephthalate with atomic force microscopy
Miscellaneous
Cell culture
Staining
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
Analytics
- TEM-EDX Measurements
- GC-MS Measurements
- Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)
