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Team:SDU-Denmark/labwork/Protocols/gel
From 2012.igem.org
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<h2>Construct a gel - Medium size</h2> | <h2>Construct a gel - Medium size</h2> | ||
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1) Weigh 0.8g agarose </br></br> | 1) Weigh 0.8g agarose </br></br> | ||
2) Mix with 100ml TBEx1 solution </br></br> | 2) Mix with 100ml TBEx1 solution </br></br> | ||
Latest revision as of 21:15, 26 September 2012
| mRNA Isolation | PCR | Miniprep | Check Digest |
| 3A-Assembly | Colony-PCR | Transformation | Gel-electrophoresis |
| Reverse Transcriptase | Mutagenesis | PCR-,gel clean-up |
Gel Electrophoresis
Construct a gel - Medium size
1) Weigh 0.8g agarose 2) Mix with 100ml TBEx1 solution 3) Heat in a microwave until all the agarose is dissolved and the solution is clear. 4) Cool it down a bit before adding Ethidium Bromide (warning: toxic) 5) Pour the solution into a gel casting mold and use a comb of desired size. 6) Run at 100V for 40 minutes.
