Team:SDU-Denmark/labwork/Protocols/gel

From 2012.igem.org

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<h2>Construct a gel  - Medium size</h2>
<h2>Construct a gel  - Medium size</h2>
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1. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Weigh 0.8g agarose  </br></br>
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1) Weigh 0.8g agarose  </br></br>
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2. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Mix with 100ml TBEx1 solution </br></br>
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2) Mix with 100ml TBEx1 solution </br></br>
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3. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Heat in a microwave until all the agarose is dissolved and the solution is clear.</br></br>
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3) Heat in a microwave until all the agarose is dissolved and the solution is clear.</br></br>
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4. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cool it down a bit before adding Ethidium Bromide (warning: toxic)</br></br>
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4) Cool it down a bit before adding Ethidium Bromide (warning: toxic)</br></br>
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5. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Pour the solution into a gel casting mold and use a comb of desired size.</br></br>
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5) Pour the solution into a gel casting mold and use a comb of desired size.</br></br>
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6. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Run at 100V for 40 minutes.     
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6) Run at 100V for 40 minutes.     
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Revision as of 21:14, 26 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenesis PCR-,gel clean-up

Gel Electrophoresis

Construct a gel - Medium size


1) Weigh 0.8g agarose

2) Mix with 100ml TBEx1 solution

3) Heat in a microwave until all the agarose is dissolved and the solution is clear.

4) Cool it down a bit before adding Ethidium Bromide (warning: toxic)

5) Pour the solution into a gel casting mold and use a comb of desired size.

6) Run at 100V for 40 minutes.

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