Team:HKUST-Hong Kong/Glossary
From 2012.igem.org
Line 372: | Line 372: | ||
<p><strong><i>lytC</i></strong> – Gene that codes for the LytC hydrolase.</p> | <p><strong><i>lytC</i></strong> – Gene that codes for the LytC hydrolase.</p> | ||
<p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins. </p> | <p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins. </p> | ||
- | <p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for | + | <p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT 29 colon carcinoma cells.</p> |
<p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p> | <p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p> | ||
<p><strong><em>PxylA</em></strong> – The region containing the sigma A binding region for the xylose inducible promoter.</p> | <p><strong><em>PxylA</em></strong> – The region containing the sigma A binding region for the xylose inducible promoter.</p> |
Revision as of 19:37, 26 September 2012
Glossary
Signal peptide – transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.
YbdN – Signal peptide of protein YbdN in Bacillus subtilis.
ybdN – Gene that codes for YbdN.
YdjM – Signal peptide from protein YdjM in Bacillus subtilis.
ydjM – Gene that codes for YdjM.
BMP2 – Bone morphogenetic protein 2.
LytC – B. subtilis inherent cell-wall associated hydrolase mediating cell-wall turnover.
lytC – Gene that codes for the LytC hydrolase.
(EAAAK)n type helical linker – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.
RPMrel – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT 29 colon carcinoma cells.
FLAG™ – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.
PxylA – The region containing the sigma A binding region for the xylose inducible promoter.
XylR – The transcriptional regulator for PxylA promoter.
Ptms – A low efficient constitutive promoter in E. coli and B. subtilis.
EndoA – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).
ydcE – Gene that codes for the EndoA endoribonuclease.
YdcD – Antidote that counteracts the function of EndoA.
ydcD (endB) – Gene that codes for the YdcD antidote.
Project
Wet Lab
Human Practice