Team:SDU-Denmark/labwork/Protocols/gel

From 2012.igem.org

(Difference between revisions)
Line 263: Line 263:
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen">Mutagenisis</a></td>
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen">Mutagenisis</a></td>
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td>
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td>
-
<td>Content</td>
+
 
</tr>
</tr>
</table>
</table>

Revision as of 17:19, 26 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenisis PCR-,gel clean-up

Gel Electrophoresis

Construct a gel - Medium size


1.      Weigh 0.8g agarose

2.      Mix with 100ml TBEx1 solution

3.      Heat in a microwave until all the agarose is dissolved and the solution is clear.

4.      Cool it down a bit before adding Ethidium Bromide (warning: toxic)

5.      Pour the solution into a gel casting mold and use a comb of desired size.

6.      Run at 100V for 40 minutes.

Retrieved from "http://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/gel"