Team:Slovenia/ModelingInteractiveSimulations

From 2012.igem.org

(Difference between revisions)
Line 428: Line 428:
       <b>INPUT VECTORS DOSAGE</b> <br/>
       <b>INPUT VECTORS DOSAGE</b> <br/>
        
        
 +
     
 +
      <p>     
 +
      <!-- inline figure 1 -->
 +
      <table class="inliner" style="width:50%;">
 +
      <tbody  class="inliner">
 +
      <tr class="inliner"><td class="inliner"><img class="inliner" src="https://static.igem.org/mediawiki/2012/f/f1/Svn12_interactive_scheme-01.png"/></td></tr>
 +
      .</td></tr>
 +
      </tbody>
 +
      </table>
 +
      <!-- end figure 1-->
        
        
       <table class="normal">
       <table class="normal">
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       </tbody>
       </tbody>
       </table>
       </table>
-
       <font color="red">(Enter dosages for corresponding plasmids.)</font><br /><br />
+
       <font color="blue">(Enter dosages for corresponding plasmids.)</font><br /><br />
 +
      </p>
        
        
 +
     
 +
      <div style="clear:both;"></div>
        
        
        
        
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       <input type="checkbox" name="req" value="1" /> Equal transcription factors
       <input type="checkbox" name="req" value="1" /> Equal transcription factors
       <br/>
       <br/>
-
       <font color="red">(Transcription factors will alter transcription with equal rates)</font><br /> <br />
+
       <font color="blue">(Transcription factors will alter transcription with equal rates)</font><br /> <br />
        
        
       <hr/>
       <hr/>
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       </table>
       </table>
        
        
-
       <font color="red">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font><br />
+
       <font color="blue">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font><br />
       <br />
       <br />
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       <hr />
       <hr />
       Time: <input type="text" name="time" size="5" value="15000" onKeyPress="return numbersonly(this, event)" maxlength="7"/> min<br />
       Time: <input type="text" name="time" size="5" value="15000" onKeyPress="return numbersonly(this, event)" maxlength="7"/> min<br />
-
       <font color="red">(Simulation running time)</font><br /><br />
+
       <font color="blue">(Simulation running time)</font><br /><br />
       <input type="submit" value="SIMULATE" /> <br />
       <input type="submit" value="SIMULATE" /> <br />
-
       <font color="red">(The results will be shown in a new browser window/tab after few seconds. Have fun.)</font><br /><br />
+
       <font color="blue">(The results will be shown in a new browser window/tab after few seconds. Have fun.)</font><br /><br />
     </form>  
     </form>  
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       <input type="hidden" name="check_submit" value="1"/>  
       <input type="hidden" name="check_submit" value="1"/>  
       <b>INPUT VECTORS DOSAGE</b>
       <b>INPUT VECTORS DOSAGE</b>
 +
     
 +
 +
      <p>     
 +
      <!-- inline figure 1 -->
 +
      <table class="inliner" style="width:50%;">
 +
      <tbody  class="inliner">
 +
      <tr class="inliner"><td class="inliner"><img class="inliner" src="https://static.igem.org/mediawiki/2012/a/a4/Svn12_interactive_scheme-02.png"/></td></tr>
 +
      .</td></tr>
 +
      </tbody>
 +
      </table>
 +
      <!-- end figure 1-->
        
        
       <table class="normal">
       <table class="normal">
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         </tbody>
         </tbody>
         </table>
         </table>
-
       <font color="red">(Enter dosages for corresponding plasmids.)</font><br /><br />
+
       <font color="blue">(Enter dosages for corresponding plasmids.)</font><br /><br />
 +
      </p>
 +
      <div style="clear:both;"></div>
 +
     
        
        
       <hr/>
       <hr/>
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       <br />
       <br />
       <input type="checkbox" name="req" value="1" /> Equal transcription factors <br />
       <input type="checkbox" name="req" value="1" /> Equal transcription factors <br />
-
       <font color="red">(Transcription factors will alter transcription with equal rates)</font><br /><br />
+
       <font color="blue">(Transcription factors will alter transcription with equal rates)</font><br /><br />
       <hr/>
       <hr/>
       <b>INDUCERS</b> <br/>
       <b>INDUCERS</b> <br/>
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       </tr>
       </tr>
       </table>
       </table>
-
       <font color="red">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font>
+
       <font color="blue">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font>
       <br />
       <br />
       <br />
       <br />
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       <b>SIMULATION</b> <br/>
       <b>SIMULATION</b> <br/>
       Time: <input type="text" name="time" size="5" value="20000"/> min<br />
       Time: <input type="text" name="time" size="5" value="20000"/> min<br />
-
       <font color="red">(Simulation running time)</font><br />
+
       <font color="blue">(Simulation running time)</font><br />
       <br />
       <br />
        
        
       <input type="submit" value="SIMULATE" /><br/>
       <input type="submit" value="SIMULATE" /><br/>
-
       <font color="red">(The results will be shown in a new browser window/tab after few seconds. Have fun.)</font>
+
       <font color="blue">(The results will be shown in a new browser window/tab after few seconds. Have fun.)</font>
     </form>  
     </form>  

Revision as of 11:53, 26 September 2012


Quantitative model interactive simulations


Based on experimental data, we constructed what we refer to as a quantitative model. Please see Quantitative and stability model for details.



Mutual repressor switch

INPUT VECTORS DOSAGE

.
ConstructDosage
[A]_pCMV_TAL-B:KRAB_BFP ng
[B]_pCMV_TAL-A:KRAB_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB ng
pCMV_[ETR]_TAL-A:KRAB ng
pCMV_PIP:KRAB ng
pCMV_E:KRAB ng
(Enter dosages for corresponding plasmids.)

TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)

INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION
Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)


Positive feedback loop switch

INPUT VECTORS DOSAGE

.
ConstructDosage
[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP ng
[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16 ng
pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16 ng
pCMV_PIP:KRAB_E:KRAB ng
(Enter dosages for corresponding plasmids.)

TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)

INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION
Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)

Retrieved from "http://2012.igem.org/Team:Slovenia/ModelingInteractiveSimulations"