Team:UC Chile/Accomplishments

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Accomplishments

Judging Criteria

We believe our team deserves the following medal: ☐Bronze ☐Silver ☐Gold

Because we met the following criteria

Bronze:

✓ Team registration
☐ Complete Judging form
✓ Team Wiki
☐ Present a poster and a talk at the iGEM Jamboree
✓ At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.

We submitted parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] wich were verified by digestion and sequencing. The functioning of these parts was extensively proved by our succesfull transformation of synechocystis, what is well documented in our results. We also characterized the functioning of KanR resistance genes [http://partsregistry.org/Part:BBa_P1003 P1003] and [http://partsregistry.org/Part:BBa_P1007 P1007] in Synechocystis PCC6803. Lastly, we quantitatively characterized the [http://partsregistry.org/Part:BBa_K325909 Luxbrick] in different temperature, o.d, glucose concentration and growth fase conditions.

Silver: In addition to the Bronze Medal requirements...

✓ Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected

Parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] worked as expected in Synechocystis double homologous recombination transformations. Part [http://partsregistry.org/Part:BBa_K743017 BBa_K743017] showed inducible expression as planned. Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] showed the right results, i.e was usefull for transformation and selection in Synechocystis.
✓Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry

Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] undergoes double homologous recombination in Synechocystis PCC6803 and conferes kanamycin resistance. Other sequences can be added to it and its effectiveness remains as shown in [http://partsregistry.org/Part:BBa_K743005 BBa_K743005]

Gold: In addition to the Bronze and Silver Medal requirements, any one or more of the following:

☐ Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year) and enter this information in the Registry (in the “Experience” section of that BioBrick’s Registry entry), and don't forget to create a new registry page for the improved part.

☐ Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.
✓Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation. We proposed a new and simple biosafety strategy designed to be easily adopted in all biobricks. It will need minimal imputs. For more information please check our biosafety section. Furthermore, we organized a Synthetic Biology workshop with worldwide renown investigators in the field. This will be the first occasion of its kind in Chile and we are sure its going to be a milestone in the history of SynBio in our country.