"
Team:Freiburg/Project
From 2012.igem.org
(Difference between revisions)
Pablinitus (Talk | contribs) (→Project) |
(→Project) |
||
| Line 12: | Line 12: | ||
We hope you will learn something about our project and the power of TAL effectors. | We hope you will learn something about our project and the power of TAL effectors. | ||
| + | == [[Team:Freiburg/Project/Overview|0. Introduction]]== | ||
| + | <p><div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> | ||
| + | You don't know what TAL effectors actually are? We reviewed the recent literature for you, to give you a quick overview of this exciting new field of research.</div> | ||
| - | == [[Team:Freiburg/Project/Overview|1. | + | == [[Team:Freiburg/Project/Overview|1. Golden Gate Standard]]== |
| - | < | + | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> Assembling multiple gene constructs in frame without leaving scars is not possible with existing iGEM standards. We therefore introduce the new Golden Gate <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> Standard that is fully compatible with RFC 10.</div> |
| + | ==[[Team:Freiburg/Project/Vektor|2. The TAL Vector]]== | ||
| - | ==[[Team:Freiburg/Project/Tal| | + | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Targeting a sequence and not doing something to it, is like throwing mechanics at your car. Your car will </div> |
| + | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">not get any better only the mechanics will get mad. Because we know this, we bring the tools you </div> | ||
| + | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">need to actually work with DNA.To make it even more easy these tools are deliverd already inside the </div> | ||
| + | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">final TAL backbone, just add the sequence and you're ready.</div> | ||
| + | |||
| + | == [[Team:Freiburg/Project/Overview|3. GATE Assembly Kit]]== | ||
| + | |||
| + | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;"> We have invented a super-fast, super-easy and super-cheap Method for custom TAL effector construction. Learn about the theory behind the TAL effector <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">toolkit, how we created it and why we choose this design.</div> | ||
| + | |||
| + | |||
| + | ==[[Team:Freiburg/Project/Tal|4. Using the Toolkit]]== | ||
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Create your own is the inoffical motto of our toolkit. Team freiGEM knows how to use it, but </div> | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">Create your own is the inoffical motto of our toolkit. Team freiGEM knows how to use it, but </div> | ||
| Line 24: | Line 38: | ||
| - | ==[[Team:Freiburg/Project/Robot| | + | ==[[Team:Freiburg/Project/Robot|4. The future TAL projects]]== |
<div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">TAL effectors are so new that we cant even talk about a history of TAL, but we should talk about</div> | <div style="color: #1C649F; font-size: 14px;font-family: Gill Sans MT; text-indent:30px;">TAL effectors are so new that we cant even talk about a history of TAL, but we should talk about</div> | ||
| Line 31: | Line 45: | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
==[[Team:Freiburg/Project/Experiments|5. Experiments]]== | ==[[Team:Freiburg/Project/Experiments|5. Experiments]]== | ||
Revision as of 01:56, 26 September 2012
Project
Overall project
Since April 2012, we are working in the field of so called TAL effectors in our lab. TAL effectors are molecular components that are fused to DNA binding proteins in order to restrict their acticvity to specific loci in a genome. In particular, we develop a strategy to cheaply create novel TAL effectors in an extremely short period of time. This new method will enable us to test numerous novel effectors in human cells and to assess their therapeutic potential for genetic diseases, epigenetic alterations and cancer.
We hope you will learn something about our project and the power of TAL effectors.
0. Introduction
You don't know what TAL effectors actually are? We reviewed the recent literature for you, to give you a quick overview of this exciting new field of research.
1. Golden Gate Standard
Assembling multiple gene constructs in frame without leaving scars is not possible with existing iGEM standards. We therefore introduce the new Golden Gate
Standard that is fully compatible with RFC 10.
2. The TAL Vector
Targeting a sequence and not doing something to it, is like throwing mechanics at your car. Your car will
not get any better only the mechanics will get mad. Because we know this, we bring the tools you
need to actually work with DNA.To make it even more easy these tools are deliverd already inside the
final TAL backbone, just add the sequence and you're ready.
3. GATE Assembly Kit
We have invented a super-fast, super-easy and super-cheap Method for custom TAL effector construction. Learn about the theory behind the TAL effector
toolkit, how we created it and why we choose this design.
4. Using the Toolkit
Create your own is the inoffical motto of our toolkit. Team freiGEM knows how to use it, but
Team freiGEM wants you to know it. Read this instruction and start working with the toolkit yourself
4. The future TAL projects
TAL effectors are so new that we cant even talk about a history of TAL, but we should talk about
their future. Our toolkit is not just easy to use but also its easy to automatable, we can proof that our kit is
easiely workable by a robot
5. Experiments
What is building without testing, and the mother of all tests is the biological experiment.
You dont believe in our kit like we do? Look how we tested it, get inside on the experiment design and the
theory behind the methods.
6. Results
But where is the proof? Here is the proof! Get data on our toolkit, learn how to read sequencing
and see the results of trials with the toolkit.
