Team:XMU-China

From 2012.igem.org

(Difference between revisions)
Line 96: Line 96:
color: #09C;
color: #09C;
}
}
-
#more{
+
 
-
font-size:12px;
+
-
text-decoration: underline;
+
-
}
+
</style>
</style>
 +
<script src="Scripts/swfobject_modified.js" type="text/javascript"></script>
</head>
</head>
Line 114: Line 112:
  <div class="tabcon">
  <div class="tabcon">
   <p>  XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device&#8230;
   <p>  XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device&#8230;
-
   <hr>
+
   <hr><a href="https://2012.igem.org/Template:Team:XMU-China/abstract">[see more]</a></div>
-
  <a href="https://2012.igem.org/Template:Team:XMU-China/abstract">[more]</a></div>
+
   
   
   
   
Line 134: Line 131:
Design:
Design:
We did some card design and drew some drafts.<hr>
We did some card design and drew some drafts.<hr>
-
<a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[more]</a>
+
<a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[see more]</a>
</div>
</div>
</div>
</div>
Line 141: Line 138:
      
      
     <!--device description-->
     <!--device description-->
-
     <td height="361" >
+
     <td height="auto" >
     <div id="device_description" >
     <div id="device_description" >
       <p style=" font-size:24px; color:#A10066;"  >
       <p style=" font-size:24px; color:#A10066;"  >
-
         DEVICE DESCRIPTION</p>
+
         DEVICE DEMONSTRATION</p>
-
       <p style=" font-size:28px; color:#004f7c">E.LUMOLI DISPLAYER</p>
+
       <p style=" font-size:28px; color:#004f7c">E.LUMOLI DISPLAYER<br>
-
      <p style=" font-size:18px;"><img src="https://static.igem.org/mediawiki/2012/a/a4/Device.png
+
        <object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" width="600" id="FlashID" title="device">
-
" width="133" height="141" style="float:right; "/>how to use balabala how 
+
          <param name="movie" value="flash/2Ddevice.swf">
-
        to use balabala how to
+
          <param name="quality" value="high">
-
        use balabala how to use
+
          <param name="wmode" value="opaque">
-
        balabala how to how to
+
          <param name="swfversion" value="6.0.65.0">
-
         use balabala how to use
+
          <!-- 此 param 标签提示使用 Flash Player 6.0 r65 和更高版本的用户下载最新版本的 Flash Player。如果您不想让用户看到该提示，请将其删除。 -->
-
         balabala how to use
+
          <param name="expressinstall" value="Scripts/expressInstall.swf">
-
    balabala how to use balabala how to use how to use balabala how to use how to use balabala </p></div>
+
          <param name="PLAY" value="false">
 +
          <!-- 下一个对象标签用于非 IE 浏览器。所以使用 IECC 将其从 IE 隐藏。 -->
 +
          <!--[if !IE]>-->
 +
          <object type="application/x-shockwave-flash" data="flash/2Ddevice.swf" width="600" height="480">
 +
            <!--<![endif]-->
 +
            <param name="quality" value="high">
 +
            <param name="wmode" value="opaque">
 +
            <param name="swfversion" value="6.0.65.0">
 +
            <param name="expressinstall" value="Scripts/expressInstall.swf">
 +
            <param name="PLAY" value="false">
 +
            <!-- 浏览器将以下替代内容显示给使用 Flash Player 6.0 和更低版本的用户。 -->
 +
            <div>
 +
              <h4>此页面上的内容需要较新版本的 Adobe Flash Player。</h4>
 +
              <p><a href="http://www.adobe.com/go/getflashplayer"><img src="http://www.adobe.com/images/shared/download_buttons/get_flash_player.gif" alt="获取 Adobe Flash Player" width="112" height="33" /></a></p>
 +
            </div>
 +
            <!--[if !IE]>-->
 +
          </object>
 +
          <!--<![endif]-->
 +
         </object>
 +
         <br>
 +
      </p>
 +
     
     <!--device description end-->
     <!--device description end-->
     </td>
     </td>
Line 163: Line 181:
<td id="title" width="300">Immobilization</td>
<td id="title" width="300">Immobilization</td>
<td id="title" width="300">Time delay</td></tr>
<td id="title" width="300">Time delay</td></tr>
-
<tr><td id="rb">Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.<a id="more" href="https://2012.igem.org/Template:Team:XMU-China/digitaldisplay">[more]</a></td>
+
<tr><td id="rb">Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.</td>
-
<td id="rb">Immobilization of Fluorescent <I>E.coli</I> cells .Design A Bioreactor for Numeric Display<a id="more" href="https://2012.igem.org/Template:Team:XMU-China/cellimmobilization">[more]</a></td>
+
<td id="rb">Immobilization of Fluorescent <I>E.coli</I> cells .Design A Bioreactor for Numeric Display</td>
-
<td><p>Testing the delay of time for GFP expression when we use different strength of RBS<a id="more" href="https://2012.igem.org/Template:Team:XMU-China/timedelay">[more]</a></p></td></tr>
+
<td><p>Testing the delay of time for GFP expression when we use different strength of RBS</p></td></tr>
</table></p>
</table></p>
-
<br><br>
+
<br><br><br>
 +
<script type="text/javascript">
 +
swfobject.registerObject("FlashID");
 +
</script>
</body>
</body>

Revision as of 07:48, 25 September 2012

XMU



homepage

ABSTRACT
PROGRESS

XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device…

[see more]
Sunday, August 26th
Digital Display: Ligated 18AI and RETPcPb and put the ligation product in 16ºC overnight for the transformation in the next day.
Time Delay: Goal: To do the ligation of pBAD-RBS0.6 and pBAD-RBS0.07. Isolate plasmids and ran on a gel. The result for analysis was correct. Cause the bands of pBAD hardly to be seem, we increased the volume of pBAD when ligation.
Fluorescence Test: We changed the abrabinose concentration from high levels to lower ones according to last fluorescence measurements of PBADRLT. Besides, we took some kinetic curves in all concentration levels we had taken before for comparison. The analysis of these data will help us improve our efficiency in the coming fluorescence measurements
Design: We did some card design and drew some drafts.
[see more]

DEVICE DEMONSTRATION

E.LUMOLI DISPLAYER

此页面上的内容需要较新版本的 Adobe Flash Player。

获取 Adobe Flash Player

Digital Display Immobilization Time delay
Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently. Immobilization of Fluorescent E.coli cells .Design A Bioreactor for Numeric Display

Testing the delay of time for GFP expression when we use different strength of RBS




Retrieved from "http://2012.igem.org/Team:XMU-China"