Team:ETH Zurich/Results

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==== Results ====
==== Results ====
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===== SDS PAGE =====
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===== Repression studies =====
[[File:Uvrr.jpg|frameless|500px|center]]
[[File:Uvrr.jpg|frameless|500px|center]]
Colonies were cultivated in the dark in the TECAN plate reader during XXX hours and YYY was measured during that time. It turned out that the fusion strategy Z. (full UVR8 directly fused to dTetR) was the best one.
Colonies were cultivated in the dark in the TECAN plate reader during XXX hours and YYY was measured during that time. It turned out that the fusion strategy Z. (full UVR8 directly fused to dTetR) was the best one.

Revision as of 22:32, 24 September 2012

Eth ecolipseeth logo.png
Eth igem logo.png

Contents

Results

LovTAP

Results

Lovv.jpg

Plans

UVR8

Results

SDS PAGE
Repression studies
Uvrr.jpg

Colonies were cultivated in the dark in the TECAN plate reader during XXX hours and YYY was measured during that time. It turned out that the fusion strategy Z. (full UVR8 directly fused to dTetR) was the best one.

Plans

In a next step we plan to measure also the derepression of the UVR8 dTetR fusion by screening its UV response with the plate reader and carry out single cell analysis by FACS and finally join UVR8 and pABA systems/RecA and show increased E.coli UV-B tolerance (sun cream properties).

UVR8 fusion purification: In vitro studies

Find constants for: Dimerization Monomerization DNA binding etc.


p-ABA generator

Results

agarose gel
Eth ladder paba.jpg
Ladder, A NheI/PstI, A XbaI/PstI, B NheI/PstI, B XbaI/PstI

As the promoter <partinfo>BBa_J23100</partinfo> has two NheI restriction sites, construct A is cut twice (lane 2) while the promoterless construct B is cut only once (lane 4).

Plans

Cloning of B downstream of 1. the Tet promoter and 2. the PL dual input promoter <partinfo>BBa_K909011</partinfo> containing the TetO1 and LacO1 operator sites.





References

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