"
Team:NTNU Trondheim/Protocols
From 2012.igem.org
(→Antibiotics) |
(→Antibiotics) |
||
| Line 23: | Line 23: | ||
Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium | Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium | ||
Chloramphenicol media concentration: 25 ug/mL = 0.25 mL stock solution/L medium | Chloramphenicol media concentration: 25 ug/mL = 0.25 mL stock solution/L medium | ||
| + | |||
| + | When transforming low copy number plasmids, it may be necessary to reduce the antibiotic to allow for the reduced antibiotic resistance of the tranformant. | ||
Revision as of 14:17, 21 June 2012
This is a list of recipes and protocols used by the team.
Contents |
Transformation
Official iGEM transformation protocol: http://partsregistry.org/Help:Protocols/Transformation
Recipes
Growth media
LB-medium (LB-Lennox):
Antibiotics
Ampicillin stock solutions: 100 mg/mL dissolved in MQ water
Kanamycin stock solutions: 100 mg/mL dissolved in MQ water
Chloramphenicol: 100 mg/mL dissolved in 20 w/v % ethanol
Store at -20 C after preparation and between use.
Ampicillin media concentration: 100 ug/mL = 1 mL stock solution/L medium
Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium
Chloramphenicol media concentration: 25 ug/mL = 0.25 mL stock solution/L medium
When transforming low copy number plasmids, it may be necessary to reduce the antibiotic to allow for the reduced antibiotic resistance of the tranformant.
