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Team:NTNU Trondheim/Protocols
From 2012.igem.org
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===Antibiotics=== | ===Antibiotics=== | ||
| - | Ampicillin stock solutions: 100 mg/mL | + | Ampicillin stock solutions: 100 mg/mL dissolved in MQ water<br> |
| - | Kanamycin stock solutions: 100 mg/mL | + | Kanamycin stock solutions: 100 mg/mL dissolved in MQ water<br> |
| + | Chloramphenicol: 100 mg/mL dissolved in 20 w/v % ethanol | ||
| - | Ampicillin media concentration: 100 ug/mL = 1 mL stock solution/L medium | + | Store at -20 C after preparation and between use. |
| + | |||
| + | Ampicillin media concentration: 100 ug/mL = 1 mL stock solution/L medium<br> | ||
Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium | Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium | ||
| + | Chloramphenicol media concentration: 25 ug/mL = 0.25 mL stock solution/L medium | ||
Revision as of 14:02, 21 June 2012
This is a list of recipes and protocols used by the team.
Contents |
Transformation
Official iGEM transformation protocol: http://partsregistry.org/Help:Protocols/Transformation
Recipes
Growth media
LB-medium (LB-Lennox):
Antibiotics
Ampicillin stock solutions: 100 mg/mL dissolved in MQ water
Kanamycin stock solutions: 100 mg/mL dissolved in MQ water
Chloramphenicol: 100 mg/mL dissolved in 20 w/v % ethanol
Store at -20 C after preparation and between use.
Ampicillin media concentration: 100 ug/mL = 1 mL stock solution/L medium
Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium
Chloramphenicol media concentration: 25 ug/mL = 0.25 mL stock solution/L medium
