Team:Freiburg/Project/Experiments
From 2012.igem.org
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- | <div align="justify">To show the | + | <div align="justify">To show the functionality of our TAL protein as well as the impact of the VP 64 transcription factor fusion protein, we used a TAL-VP64 fusion construct targetting a minimal promotor coupled with EGFP. |
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- | The | + | The experiment was done with four different transfections, either no plasmid, only the TAL vector, only the test plasmid or a cotransfection of both plasmids. |
Revision as of 17:02, 24 September 2012
Gene activation
To show the functionality of our TAL protein as well as the impact of the VP 64 transcription factor fusion protein, we used a TAL-VP64 fusion construct targetting a minimal promotor coupled with EGFP.
The experiment was done with four different transfections, either no plasmid, only the TAL vector, only the test plasmid or a cotransfection of both plasmids.