Team:Goettingen/week21-3
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- | <h2><b> | + | <h2><b>V09_17 </b></h2><br> |
- | <b> | + | <b>Synthesis of cDNA and qrtPCR</b><br> |
<ul> | <ul> | ||
- | <li>Experiment: <br> | + | <li>Experiment: <br>cDNA synthesis was performed using Reverse Transcription Kit (Qiagen).<br> |
+ | qrtPCR with promoter constructs was performed, for a detailed protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Experimental_design">click here</a>.<br> | ||
+ | </li> | ||
+ | <li>Observations & Results<br> | ||
+ | CP-values of triplets showed values in a range which were useful for <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Analysis_via_the_2.E2.80.93.CE.94.CE.94CT_.28Livak.29_Method">calculation</a> of expression rates. </li> | ||
</ul> | </ul> | ||
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Revision as of 15:58, 24 September 2012
Language: English, Deutsch
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#3 Chemoreceptor Library - 21th WeekBack to overview
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