Team:GoettingenNeu

Welcome to iGEM Göttingen

In November 2011, most of us heard about iGEM competition for the first time. Erik, inspired by his former colleagues's enthusiastic reports, was looking for like-minded people at his new university that were willing to organize a whole project in the field of Synthetic Biology. He described the idea of the contest to anybody willing to listen. It did not take long to find five other interested students. Thus, the so called organization task force of the first iGEM team of the University of Göttingen was formed.

Luckily, the iGEM competition was already well known among the University´s professors and the team found his first supporters: Prof. Heinz Neumann, head of a work group specializing in Synthetic Biology, and Prof. Jörg Stülke from the department of General and Applied Microbiology. The next task was to find a project that was interesting as well as realizable. After a lot of back and forth due to extensive discussions, Prof. Neumann presented the perfect project: "Homing Coli"!

After many drawbacks, like finding a suitable laboratory to realize the project, the search for additional team members began. The new fellows were quickly found, which was the story starting point of the first iGEM team of the University of Göttingen… Below you can see our timeline.

The timeline of the iGEM team Göttingen 2012. Click on the picture for full size.

If you are interested in the <a href="https://2012.igem.org/Team:Goettingen/iGEM">iGEM competition</a>, the BioBrick concept, and – most importantly – <a href="https://2012.igem.org/Team:Goettingen/Project">our project</a>, take a look around. Here, you will find everything you need to know about "Homing Coli" and you will have the opportunity to gather knowledge about chemotaxis in general!
Visit our <a href="https://2012.igem.org/Team:Goettingen/Team">team site</a> to learn more about us and our supporters and advisors! And, last but not least, do not forget to check out our mascot <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">"Flash Coli"</a>! He always has some interesting stories to tell and needs YOUR help to fight the horrible phages!

Feel free to browse this page!

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Homing Coli

Escherichia coli is a commonly used bacterial model organism. It has lots of beneficial traits, e.g. a short generation time and it can be easily manipulated. Most E. coli strains that are used in laboratories do not exhibit high motility. The crucial element for motility is the flagellum, which is rotated by a molecular motor within the cell wall. Consequently, these are reduced in cultivated E. coli strains.

Our goal is to create an E. coli strain with increased swimming motility on special

agar plates. Therefore, we will overexpress regulators and parts of the E. coli flagellum in cultivated strains in order to enhance bacterial swimming ability. The fastest E. coli strains will be selected and
further improved. At the same time we will then be able to create an effective motility-selection
 method.         

Now, you are probably wondering what the advantage of a fast E. coli might be. The beneficial fast phenotype can be combined with the ability of this bacterium to sense specific compounds in their environments. Chemoreceptors enable it to move towards or along gradients of such substances. We will use PCR-based site directed mutagenesis on the E. coli Tar receptor. The combination of speed and chemotaxis allows us to identify E. coli strains, which can sense interesting compounds. Thereby, an easy method for the detection of pollutants, toxins or even tumors could be provided.

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