Team:Goettingen/week7-2

From 2012.igem.org

(Difference between revisions)
Line 527: Line 527:
<tr bordercolor="black" valign="top">
<tr bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
-
 
<h2><b>V06_11 </b></h2><br>
<h2><b>V06_11 </b></h2><br>
<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
-
 
+
<ul>
-
        <ul>
+
<li>Experiment: <br>
-
                <li>Experiment: <br>
+
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
-
 
+
</ul>
-
        </ul>
+
<br>
 +
<b>V06_11_2 Chemical retransformation of the new <i>flhDC</i>-promoter constructs into <i>E. coli</i> (DH10B)</b><br>
 +
<ul>
 +
<li>Experiment:  <br>
 +
For the chemical retransformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed. The following constructs were transformed into <i>E. coli</i>:<br>
<br>
<br>
-
 
-
 
-
<b>V06_11_2 Chemical retransformartion of the new <i>flhDC</i>-promoter constructs into <i>E. coli</i> (DH10B)</b><br>
 
-
        <ul>
 
-
                <li>Experiment:  <br>
 
-
For the chemical retransformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> for transformation was followed. The following constructs were transformed into <i>E. coli</i>:<br>
 
20G - #1 <br>
20G - #1 <br>
20G - #2 <br>
20G - #2 <br>
Line 555: Line 551:
18K - #2 <br>
18K - #2 <br>
18K - #3 <br></li>
18K - #3 <br></li>
-
                <li>Observations & Results: <br>
+
<br>
 +
<li>Observations & Results: <br>
The retransformation was successful since all plates showed numeours colonies except the negative control.
The retransformation was successful since all plates showed numeours colonies except the negative control.
-
    </ul>
+
</ul>
-
 
+
-
 
+
<br></td></tr>
<br></td></tr>
</table>
</table>
Line 573: Line 568:
<li>Experiment: <br>
<li>Experiment: <br>
In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>
In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>
-
20E - #2 <br>
+
<br>
-
20G - #1 <br>
+
- 20E - #2 <br>
-
2G - #1 <br>
+
- 20G - #1 <br>
-
20I - #2 <br>
+
- 2G - #1 <br>
-
18M - #2 <br>
+
- 20I - #2 <br>
-
18O - #2 <br>
+
- 18M - #2 <br>
-
18K - #1 <br>
+
- 18O - #2 <br>
-
18C - #2 <br>
+
- 18K - #1 <br>
 +
- 18C - #2 <br>
 +
<br>
<br></td></tr>
<br></td></tr>
</table>
</table>
Line 596: Line 593:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li></ul>
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li></ul>
<br>
<br>
-
<b>V06_13_2 Chemical transformartion of pBAD-<i>sfGFP</i> into <i>E. coli</i> (DH10B)</b><br>
+
<b>V06_13_2 Chemical transformation of pBAD-<i>sfGFP</i> into <i>E. coli</i> (DH10B)</b><br>
<ul>
<ul>
<li>Experiment:  <br>
<li>Experiment:  <br>

Revision as of 09:38, 21 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week7-2"