Team:Goettingen/week10-3

(Difference between revisions)

Revision as of 09:28, 20 September 2012

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Startpoint of Saturated mutagenesis experiment!

Observations and results:
According to the agarose gel the PCR worked well. A band at the expected size of 3769 bp was observed for both reactions.

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 TARY149F150T154Fw: <br>gcgcaGGAAAGGTCTCAGGAGCTnnNnnNGCTCAGCCAnnNCAGGGAATGCAAAATGCAATGGGCGAAG<br>
 TARY149F150T154Rv: <br>gcgcaGAGTAGGTCTCACTCCAGTATTGCCATAATCTAGGTAATC<br><br>
-We firstly did a test PCR with both primer pairs to verify the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">mutagenesis PCR protocol</a> and conditions.
+We firstly did a test PCR with both primer pairs to verify the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">mutagenesis PCR protocol</a> and conditions. As a template we use pSB1C3_TAR_QC_18C since this promoter is the strongest among the promoter constructs we use.
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+</ul>
+<ul>
+<li>Observations and results: <br>According to the agarose gel the PCR worked well. A band at the expected size of 3769 bp was observed for both reactions.
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 </ul>