Team:Goettingen/week18-1
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- | <h2><b> | + | |
- | <b> | + | <h2><b>V09_1 </b></h2><br> |
+ | <b>V09_01_1: Selection of the BL21 <i>tar</i> library, first approach, fourth round</b><br> | ||
<ul> | <ul> | ||
- | <li>Experiment: <br> | + | <li>Experiment: <br>The first approach of the selction of clones that move directly towards our chosen attractants was selected for a fourth time, bacause no chemotaxis could be observed in the third round.</li> |
+ | <li>Experimental procedure: <br>The selection was conducted as described in the methods, but small petridishes (9 cm) were used.</li> | ||
+ | <li>Observation: <br>On the next day swimming could be observed and the plated were treated as described in the protocol "plating of selected clones"</li><br> | ||
+ | <b>V09_01_2: Separation assay</b><br> | ||
+ | <ul> | ||
+ | <li>Experiment: <br>One of our goals was to invent es protocol that allows the separation of two different fast strains using the anitibiotics marker on an inserted plasmid. </li> | ||
+ | <li>Experimental procedure: <br>The selection was conducted as described in the methods, but small petridishes (9 cm) were used.</li> | ||
+ | <li>Observation: <br>On the next day swimming could be observed and the plated were treated as described in the protocol "plating of selected clones"</li><br> | ||
+ | |||
</ul> | </ul> | ||
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Revision as of 20:40, 19 September 2012
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