Team:Goettingen/week7-2

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Revision as of 14:16, 18 September 2012

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V06_11_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
20G - #1
20G - #2
2G - #1
2G - #2
2G - #3
20I - #2
20I - #3
18O - #2
18O - #3
18K - #1
18K - #2
18K - #3
Observations & Results:
The retransformation was successful since all plates showed numeours colonies except the negative control.

Preparation of over night cultures

V06_13_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_13_2 Chemical transformartion of pBAD-sfGFP into E. coli (DH10B)

Experiment:
For the chemical transformation the standard protocol was followed.
Observations & Results:
The transformation was successful since all plates showed numeours colonies except the negative control.

Preparation of over night cultures

Experiment:
Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.

Miniprep of pBAD-sfGFP

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 542: / Line 542: @@
           <ul>
                   <li>Experiment:  <br>
-For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into <i>E. coli</i>:<br>
+For the chemical retransformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> for transformation was followed. The following constructs were transformed into <i>E. coli</i>:<br>
 G - #1 <br>
 G - #2 <br>
@@ Line 556: / Line 556: @@
 K - #3 <br></li>
                   <li>Observations & Results: <br>
-The retransformation was successful since all plates showed numeours colonoes except the negative control.
+The retransformation was successful since all plates showed numeours colonies except the negative control.
      </ul>
@@ Line 599: / Line 599: @@
 <ul>
 <li>Experiment:  <br>
-For the chemical transformation the standard protocol was followed. <br>
+For the chemical transformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed. <br>
 <li>Observations & Results: <br>
-The transformation was successful since all plates showed numeours colonoes except the negative control.</li>
+The transformation was successful since all plates showed numeours colonies except the negative control.</li>
 </ul><br>