Team:Goettingen/week21-2

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#2 Speed Improvement - 21st week

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V09_17

V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and pSB1C3 followed by ligation

Experiment:
In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .

V09_17_2 Preparation of over night cultures

Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (Mg1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used:

- 20E_flhDC
- 18C_flhDC
- 20I_flhdc
- 20E_motA
- 18C_motA
- 20E_motB
- 18C_motB

Versuchsnummer

Titel

Experiment:
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@@ Line 531: / Line 531: @@
 <td width="900" bordercolor="black" valign="top">
 <h2><b>V09_17 </b></h2><br>
-<b>V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and Psb1c3 followed by ligation</i></b><br>
+<b>V09_17_1 Preparative double digestion of <i>flhDC</i>, <i>FliC</i>, 18K-<i>RFP</i> and pSB1C3 followed by ligation</i></b><br>
 <ul>
 <li>Experiment: <br>
-In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
+In order to clone the <i>flhDC</i>, <i>FliC</i> and 18K-<i>RFP</i> constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
           </ul>
 <br>
@@ Line 541: / Line 541: @@
 <li>Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (Mg1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used: <br>
 <br>
-- 20E_flhDC <br>
+- 20E_<i>flhDC</i> <br>
-- 18C_flhDC <br>
+- 18C_<i>flhDC</i> <br>
-- 20I_flhdc <br>
+- 20I_<i>flhdc</i> <br>
-- 20E_motA <br>
+- 20E_<i>motA</i> <br>
-- 18C_motA <br>
+- 18C_<i>motA</i> <br>
-- 20E motB <br>
+- 20E_<i>motB</i> <br>
-- 18C_motB <br></li>
+- 18C_<i>motB</i> <br></li>
 </li></ul>
 <br>