Team:Goettingen/week21-2

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Revision as of 07:37, 18 September 2012

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V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and Psb1c3 followed by ligation

Experiment:
In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .

V09_17_2 Preparation of over night cultures

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Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <ul>
 <li>Experiment: <br>
-In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a> href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
+In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
           </ul>
 <br>