Team:Goettingen/week8-3

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#3 Chemoreceptor Library - 8th Week

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V06_18

Isolation of vector backbone pSB1C3 from BioBrick distribution kit

Experiment:
The vector pSB1C3 is used as the standard vector for BioBricks in this year's iGEM competition. We transformed E. coli DH10B with pSB1C3 according to protocol and streaked these out on Chloramphenicol (CM) plates.

V06_22

V06_22_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3

Experiment:
All 8 promoter constructs + TAR_QC in J61002 and the BioBrick vector pSB1C3 were digested with XbaI and PstI according to protocol.

Observations and results:
The transformation of DH10B with pSB1C3 (see V06_18) worked well. pSB1C3 contained RFP which is now cut out.

V06_22_2 Preparation of glycerol stocks

Experiment:
Glycerol stocks (30 %) of 2 clones E. coli DH10B with pSB1C3 were prepared and stored at -80 °C.

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-<h2><b>V06_21 </b></h2><br>
+<h2><b>V06_22 </b></h2><br>
-<b>V06_21_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
+<b>V06_22_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
 <ul>
 <li>Experiment: <br>All 8 promoter constructs + TAR_QC in J61002 and the BioBrick vector pSB1C3 were digested with <i>Xba</i>I and <i>Pst</i>I according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol. </a></li>
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 </ul>
 <br>
-<b>V06_21_2 Preparation of glycerol stocks <i>E. coli</i> DH10B with pSB1C3</b><br>
+<b>V06_22_2 Preparation of glycerol stocks</b><br>
 <ul>
-<li>Experiment: <br>Glycerol stocks (30 %) were of 2 clones were prepared and stored at -80 °C.</a></li>
+<li>Experiment: <br>Glycerol stocks (30 %) of 2 clones <i>E. coli</i> DH10B with pSB1C3 were prepared and stored at -80 °C.</a></li>
 </ul>
 <ul>
-<br></td></tr>
-</table>
-<br>
-<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
-<tr bordercolor="black" valign="top">
-<td width="900" bordercolor="black" valign="top">
-<h2><b>V06_22 </b></h2><br>
-<b>Isolation of vector backbone pSB1C3 from BioBrick distribution kit</b><br>
-<ul>
-<li>Experiment: <br>The vector pSB1C3 is used as the standard vector for BioBricks in this year's iGEM competition. We transformed <i>E. coli</i> DH10B with pSB1C3 according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> and streaked these out on Chloramphenicol (CM) plates.</li>
-</ul>
 <br></td></tr>
 </table>