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Team:TU Darmstadt/Protocols/Bacterial transformation
From 2012.igem.org
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== Bacterial transformation == | == Bacterial transformation == | ||
There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are: | There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are: | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] and | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation] |
| + | and | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation] | ||
The transformation of ''E. coli'' with plasmid DNA according to those methods do require [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically]/[https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells electro]-competent cells. | The transformation of ''E. coli'' with plasmid DNA according to those methods do require [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically]/[https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells electro]-competent cells. | ||
Revision as of 21:26, 16 September 2012
Bacterial transformation
There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are:
and
The transformation of E. coli with plasmid DNA according to those methods do require chemically/electro-competent cells.
Application
References
- Praktikumsskript Biotechnologie 2012 TU Darmstadt
